Stock Michael, Salvay David, Shoss Bradley, Piggott Kisha, Culican Susan M
Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, MO.
Cornea. 2015 Nov;34(11):1523-5. doi: 10.1097/ICO.0000000000000588.
With new compounding pharmacy laws, the only economically feasible approach to using lissamine is through dye-impregnated strips. This research aims to determine the concentration of lissamine that can be obtained using a single commercially available lissamine strip. With the optimal vital staining requiring 1% concentration of lissamine, we sought to obtain this concentration using supplies in an ordinary ophthalmology clinic.
A standard curve was generated using compounded lissamine green 1% solution. Serial dilutions were made with 3 different diluents and measured using a spectrophotometer at a wavelength of 633 μm. Combinations of the number of strips, amount of solvent, and absorption time were performed to obtain a 1% solution. Cost analyses were performed to select the most economical method.
Single lissamine strips wetted with any of the diluents produced 0.17% ± 0.05% (95% confidence interval) lissamine solution, a 5-fold weaker concentration than the optimal for vital staining. Combinations of 4 strips in 200 μL (4 drops) for 1 minute and 2 strips in 200 μL for 5 minutes were found to reach concentrations of 1%. Cost analysis showed that the 2 strip/4 drops/5 minutes method costs $0.67 and the 4 strips/4 drops/1 minute method $1.27.
Use of a single lissamine strip leads to suboptimal concentrations for vital staining. With only the addition of disposable microcentrifuge tubes to the clinical setting, ophthalmologists can make 1% solutions of lissamine. This solution is both more economical and in compliance with both state and national compounding laws.
随着新的复方药房法规的出台，使用丽丝胺绿的唯一经济可行方法是通过染料浸渍试纸条。本研究旨在确定使用单一市售丽丝胺绿试纸条可获得的丽丝胺绿浓度。鉴于最佳活体染色需要1%浓度的丽丝胺绿，我们试图在普通眼科诊所利用现有材料获得该浓度。
使用配制的1%丽丝胺绿溶液生成标准曲线。用3种不同的稀释剂进行系列稀释，并在633μm波长下使用分光光度计进行测量。对试纸条数量、溶剂量和吸收时间的组合进行实验，以获得1%的溶液。进行成本分析以选择最经济的方法。
用任何一种稀释剂湿润的单个丽丝胺绿试纸条产生的丽丝胺绿溶液浓度为0.17%±0.05%（95%置信区间），比活体染色的最佳浓度弱5倍。发现4条试纸条在200μL（4滴）中浸泡1分钟以及2条试纸条在200μL中浸泡5分钟的组合可达到1%的浓度。成本分析表明，2条试纸条/4滴/5分钟的方法成本为0.67美元，4条试纸条/4滴/1分钟的方法成本为1.27美元。
使用单个丽丝胺绿试纸条会导致活体染色浓度不理想。只需在临床环境中增加一次性微量离心管，眼科医生就可以配制1%的丽丝胺绿溶液。该溶液既更经济又符合州和国家的复方法规。
