School of Optometry and Vision Science, UNSW Sydney, Sydney, Australia.
School of Optometry and Vision Science, UNSW Sydney, Sydney, Australia; Save Sight Institute, Sydney Eye Hospital, Sydney, Australia.
Cont Lens Anterior Eye. 2018 Feb;41(1):23-27. doi: 10.1016/j.clae.2017.11.002. Epub 2017 Nov 21.
To investigate the performance of lissamine green strips from different manufacturers. Additionally, the repeatability, need for sequential dye instillation and impact of repeated lid evertion on lid wiper staining were assessed.
Study 1 was a prospective, randomised cross-over study where controlled volumes of lissamine green solution prepared from strips (Biotech, Lissaver, GreenGlo, OPGreen) were instilled (right eye: single; left eye: double instillation) on five different days, with OPGreen being tested twice. Lids were everted and digital photographs taken, which were later assessed by a masked observer. Study 2 was an investigator-masked, randomised, controlled study testing the impact of single versus repeated lid evertion. Lid wiper staining was graded (0 to 3 in 0.5 steps).
Lid wiper staining differed significantly between lissamine green solutions, with GreenGlo showing the highest amount of staining, and Lissaver the least (all p>0.009). There were no differences in lid wiper staining over two days, using the OPGreen solution (all p>0.05). The number of drops instilled (single versus double) did not significantly affect lid wiper staining (all p>0.05). Repeated lid evertion increased lid wiper staining (p=0.007 when combined with double drop instillation). Light absorbance patterns and measured concentrations aligned with clinical findings.
There were significant differences in performance between lissamine green solutions. Lid wiper staining was impacted by repeated lid evertion but sequential instillation and use of the Korb grading scale provided little advantage over simpler methods Clinicians must consider this when investigating lid wipers, especially when interpreting a negative finding.
研究不同厂家生产的丽丝胺绿条的性能。此外，还评估了重复性、是否需要连续滴注染料以及反复翻转眼睑对眼睑擦拭器染色的影响。
研究 1 是一项前瞻性、随机交叉研究，在五天内，用来自不同厂家的丽丝胺绿溶液（Biotech、Lissaver、GreenGlo、OPGreen）滴注（右眼：单次；左眼：双次滴注）控制体积的溶液，并对 OPGreen 进行两次测试。然后翻转眼睑并拍摄数字照片，由一名蒙面观察者进行评估。研究 2 是一项研究者盲法、随机、对照研究，测试单次与反复翻转眼睑对眼睑擦拭器染色的影响。眼睑擦拭器染色程度（0 至 3 级，每级 0.5 级）。
丽丝胺绿溶液之间的眼睑擦拭器染色有显著差异，GreenGlo 显示出最高的染色量，而 Lissaver 最少（均 p>0.009）。在两天内，使用 OPGreen 溶液时，眼睑擦拭器染色没有差异（均 p>0.05）。滴注的滴数（单次与双次）对眼睑擦拭器染色没有显著影响（均 p>0.05）。反复翻转眼睑会增加眼睑擦拭器染色（当与双滴滴注结合时，p=0.007）。光吸收模式和测量浓度与临床发现一致。
丽丝胺绿溶液的性能存在显著差异。眼睑擦拭器染色受反复翻转眼睑影响，但连续滴注和使用 Korb 分级标准并没有比更简单的方法提供太多优势。临床医生在研究眼睑擦拭器时必须考虑到这一点，特别是在解释阴性发现时。
