Zamani Khosrow, Emami Amir, Bazargani Abdollah, Moattari Afagh
Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IR.
Rev Soc Bras Med Trop. 2015 Jul-Aug;48(4):479-82. doi: 10.1590/0037-8682-0315-2014.
The aim of this study was to detect the prevalence of the extended-spectrum beta-lactamase (ESBL)-encoding CTX-M gene in Escherichia coliisolates.
Phenotypic screening of 376 E. coli isolates for ESBL was conducted using disk diffusion. ESBL-producing isolates were tested using PCR and specific primers. The bla(CTX-M) cluster was identified using the RFLP method, and its genotype was sequenced.
From 202 ESBL-producing E. coli , 185 (91.5%) possessed CTX-M genes. CTX-M-1 subtypes were found in 98% of the isolates. The bla(CTX-M) gene was identical to CTX-M-15.
A high prevalence of CTX-M-1-producing E. coli apparently exists in Shiraz, Iran.
本研究旨在检测大肠杆菌分离株中编码超广谱β-内酰胺酶（ESBL）的CTX-M基因的流行情况。
采用纸片扩散法对376株大肠杆菌分离株进行ESBL表型筛选。对产ESBL的分离株使用PCR和特异性引物进行检测。采用RFLP方法鉴定bla(CTX-M)簇，并对其基因型进行测序。
在202株产ESBL的大肠杆菌中，185株（91.5%）携带CTX-M基因。98%的分离株中发现了CTX-M-1亚型。bla(CTX-M)基因与CTX-M-15相同。
在伊朗设拉子，产CTX-M-1的大肠杆菌明显具有较高的流行率。
