伊朗设拉子产CTX-M超广谱β-内酰胺酶大肠杆菌分离株的表型和分子特征
Phenotypic and molecular characterization of CTX-M extended-spectrum beta-lactamase-producing Escherichia coli isolates in Shiraz, Iran.
作者信息
Zamani Khosrow, Emami Amir, Bazargani Abdollah, Moattari Afagh
机构信息
Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IR.
出版信息
Rev Soc Bras Med Trop. 2015 Jul-Aug;48(4):479-82. doi: 10.1590/0037-8682-0315-2014.
INTRODUCTION
The aim of this study was to detect the prevalence of the extended-spectrum beta-lactamase (ESBL)-encoding CTX-M gene in Escherichia coliisolates.
METHODS
Phenotypic screening of 376 E. coli isolates for ESBL was conducted using disk diffusion. ESBL-producing isolates were tested using PCR and specific primers. The bla(CTX-M) cluster was identified using the RFLP method, and its genotype was sequenced.
RESULTS
From 202 ESBL-producing E. coli , 185 (91.5%) possessed CTX-M genes. CTX-M-1 subtypes were found in 98% of the isolates. The bla(CTX-M) gene was identical to CTX-M-15.
CONCLUSIONS
A high prevalence of CTX-M-1-producing E. coli apparently exists in Shiraz, Iran.
引言
本研究旨在检测大肠杆菌分离株中编码超广谱β-内酰胺酶（ESBL）的CTX-M基因的流行情况。
方法
采用纸片扩散法对376株大肠杆菌分离株进行ESBL表型筛选。对产ESBL的分离株使用PCR和特异性引物进行检测。采用RFLP方法鉴定bla(CTX-M)簇，并对其基因型进行测序。
结果
在202株产ESBL的大肠杆菌中，185株（91.5%）携带CTX-M基因。98%的分离株中发现了CTX-M-1亚型。bla(CTX-M)基因与CTX-M-15相同。
结论
在伊朗设拉子，产CTX-M-1的大肠杆菌明显具有较高的流行率。
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