Key Laboratory for Nano-Bio InterfaceDivision of Nanobiomedicine, Collaborative Innovation Center of Suzhou Nano Science and Technology, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
Small. 2015 Oct 28;11(40):5444-51. doi: 10.1002/smll.201501718. Epub 2015 Aug 27.
Circulating tumor cell (CTC) isolation has attracted a great deal of research interest in recent years. However, there are still some challenges, including purity as well as viability of the captured CTCs, resulting from nanoscale structures and inorganic nanomaterials. Here, a chitosan nanoparticle surface is first fabricated by electrospray to provide a cellular compatible interface. The "soft" substrate, further modified by polyethylene glycol (PEG) as an antifouling molecule and DNA aptamer as a specific capture molecule, has a hydrophilic nature and is capable of specific capture of viable rare CTCs from artificial white blood cell (WBC) samples. Furthermore, a subsequent in situ culture strategy based on the developed cellular compatible soft interface is introduced for further purification and proliferation of the captured rare number target cells. The WBCs are weeded out after 2 d, and after a 7 d proliferation nearly 200 MCF-7 cells are obtained from 7 target cells with more than 90% purity. This work provides a promising strategy for viable isolation and purification of rare CTCs and it has great potential for achieving clinical validity.
近年来，循环肿瘤细胞（CTC）的分离引起了广泛的研究兴趣。然而，由于纳米级结构和无机纳米材料的存在，仍然存在一些挑战，包括捕获的 CTC 的纯度和活力。在这里，首先通过电喷雾制造壳聚糖纳米颗粒表面，以提供细胞相容的界面。“柔软”的基底进一步用聚乙二醇（PEG）作为抗污分子和 DNA 适体作为特定的捕获分子进行修饰，具有亲水性，能够从人工白细胞（WBC）样品中特异性捕获有活力的稀有 CTC。此外，引入了一种基于所开发的细胞相容软界面的后续原位培养策略，用于进一步纯化和增殖捕获的稀有靶细胞。在 2 天后，剔除 WBC，经过 7 天的增殖，从 7 个靶细胞中获得了近 200 个 MCF-7 细胞，纯度超过 90%。这项工作为有活力的稀有 CTC 的分离和纯化提供了一种有前途的策略，对于实现临床有效性具有巨大的潜力。
