Veljović Katarina, Popović Nikola, Vidojević Amarela Terzić, Tolinački Maja, Mihajlović Sanja, Jovčić Branko, Kojić Milan
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Vojvode Stepe 444a, P. O. Box 23, 11010, Belgrade, Serbia,
Environ Monit Assess. 2015 Sep;187(9):599. doi: 10.1007/s10661-015-4814-x. Epub 2015 Aug 28.
Despite the number of studies on antibiotic-resistant enterococci from Serbian clinical settings, there are no data about environmental contamination with these bacteria. Thus, this study investigated the prevalence of antibiotic-resistant enterococci in Belgrade, Serbia. Enterococcus species collected from ten surface water sites, including a lake, two major river systems, and springs, were tested. Among enterococci, we found single (21.7 %), double (17.4 %), and multiple antibiotic resistance patterns (56.3 %). Vancomycin-resistant strains were not found, indicating that their abundance in Belgrade is tightly linked to clinical settings. The multiple drug-resistant strains Enterococcus faecalis, Enterococcus faecium, and Enterococcus mundtii were frequently detected in the lake during the swimming season and in the rivers near industrial zones. We confirmed the presence of ermB, ermC, ant(6)-Ia, tetM, and tetL and mutations in gyrA genes. The phylogenetic analysis of 16S rRNA gene of E. faecium isolates that harbor esp gene classified them into two groups based on high-bootstraps scores in the tree analysis. Pulsed-field gel electrophoresis analysis of antibiotic-resistant enterococci revealed genomic similarity ranging from 75 to 100 %. This study indicates the importance of anthropogenic impact to the spread of antibiotic-resistant enterococci in environmental waters of Belgrade, Serbia.
尽管有许多关于塞尔维亚临床环境中耐抗生素肠球菌的研究，但尚无关于这些细菌环境污染情况的数据。因此，本研究调查了塞尔维亚贝尔格莱德耐抗生素肠球菌的流行情况。对从十个地表水地点采集的肠球菌进行了检测，这些地点包括一个湖泊、两个主要河流系统和一些泉水。在肠球菌中，我们发现了单一耐药（21.7%）、双重耐药（17.4%）和多重耐药模式（56.3%）。未发现耐万古霉素菌株，这表明它们在贝尔格莱德的丰度与临床环境密切相关。在游泳季节的湖泊和工业区附近的河流中，经常检测到多重耐药菌株粪肠球菌、屎肠球菌和蒙氏肠球菌。我们证实了ermB、ermC、ant(6)-Ia、tetM和tetL基因的存在以及gyrA基因的突变。对携带esp基因的屎肠球菌分离株的16S rRNA基因进行系统发育分析，根据树状分析中的高自展值将它们分为两组。对抗生素耐药肠球菌的脉冲场凝胶电泳分析显示基因组相似性在75%至100%之间。本研究表明人为影响对塞尔维亚贝尔格莱德环境水体中耐抗生素肠球菌传播的重要性。
