生物物理学。对“极端电场助力酮甾类异构酶活性位点的催化作用”的评论。
BIOPHYSICS. Comment on "Extreme electric fields power catalysis in the active site of ketosteroid isomerase".
作者信息
Chen Deliang, Savidge Tor
机构信息
Jiangxi Key Laboratory of Organo-Pharmaceutical Chemistry, Gannan Normal University, Jiangxi 341000, China.
Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX 77030, USA. Department of Pathology, Texas Children's Hospital, Houston, TX 77030, USA.
出版信息
Science. 2015 Aug 28;349(6251):936. doi: 10.1126/science.aab0095. Epub 2015 Aug 27.
Abstract
Fried et al. (Reports, 19 December 2014, p. 1510) demonstrate electric field-dependent acceleration of biological catalysis using ketosteroid isomerase as a prototypic example. These findings were not extended to aqueous solution because water by itself has field fluctuations that are too large and fast to provide a catalytic effect. Given physiological context, when water electrostatic interactions are considered, electric fields play a less important role in the catalysis.
摘要
弗里德等人（《报告》，2014年12月19日，第1510页）以酮甾体异构酶作为典型示例，证明了生物催化存在电场依赖性加速作用。这些发现并未扩展到水溶液中，因为水自身存在的场波动过大且过快，无法产生催化作用。考虑到生理环境，当考虑水的静电相互作用时，电场在催化过程中所起的作用较小。
相似文献
1
BIOPHYSICS. Comment on "Extreme electric fields power catalysis in the active site of ketosteroid isomerase".生物物理学。对“极端电场助力酮甾类异构酶活性位点的催化作用”的评论。
Science. 2015 Aug 28;349(6251):936. doi: 10.1126/science.aab0095. Epub 2015 Aug 27.
2
BIOPHYSICS. Comment on "Extreme electric fields power catalysis in the active site of ketosteroid isomerase".生物物理学. 评论“极端电场在酮固醇异构酶活性部位推动催化反应”。
Science. 2015 Aug 28;349(6251):936. doi: 10.1126/science.aab1584. Epub 2015 Aug 27.
3
BIOPHYSICS. Response to Comments on "Extreme electric fields power catalysis in the active site of ketosteroid isomerase".生物物理学。对《极端电场助力酮甾类异构酶活性位点催化作用》评论的回应
Science. 2015 Aug 28;349(6251):936. doi: 10.1126/science.aab1627. Epub 2015 Aug 27.
4
Extreme electric fields power catalysis in the active site of ketosteroid isomerase.极强电场助力酮甾体异构酶活性位点的催化作用。
Science. 2014 Dec 19;346(6216):1510-4. doi: 10.1126/science.1259802.
5
A Critical Test of the Electrostatic Contribution to Catalysis with Noncanonical Amino Acids in Ketosteroid Isomerase.非典型氨基酸在酮固醇异构酶中对催化的静电贡献的关键检验。
J Am Chem Soc. 2016 Sep 14;138(36):11890-5. doi: 10.1021/jacs.6b06843. Epub 2016 Sep 1.
6
Fluctuations of Electric Fields in the Active Site of the Enzyme Ketosteroid Isomerase.酶酮固醇异构酶活性部位的电场波动。
J Am Chem Soc. 2019 Aug 14;141(32):12487-12492. doi: 10.1021/jacs.9b05323. Epub 2019 Aug 2.
7
QM/MM modelling of ketosteroid isomerase reactivity indicates that active site closure is integral to catalysis.QM/MM 建模研究酮甾体异构酶反应性表明活性位点的封闭是催化的必要条件。
FEBS J. 2013 Jul;280(13):3120-31. doi: 10.1111/febs.12158. Epub 2013 Feb 27.
8
A Preorganized Electric Field Leads to Minimal Geometrical Reorientation in the Catalytic Reaction of Ketosteroid Isomerase.预组织电场导致酮甾体异构酶催化反应中最小的几何重排。
J Am Chem Soc. 2020 Jun 3;142(22):9993-9998. doi: 10.1021/jacs.0c00383. Epub 2020 May 19.
9
Ketosteroid isomerase provides further support for the idea that enzymes work by electrostatic preorganization.酮甾体异构酶为酶通过静电预组织起作用的观点提供了进一步的支持。
Proc Natl Acad Sci U S A. 2010 Mar 2;107(9):4075-80. doi: 10.1073/pnas.0914579107. Epub 2010 Feb 11.
10
Structure and enzymology of Delta5-3-ketosteroid isomerase.Δ5-3-酮甾体异构酶的结构与酶学
Curr Opin Struct Biol. 2001 Dec;11(6):674-8. doi: 10.1016/s0959-440x(01)00268-8.
引用本文的文献
1
DFT study on capture using boron, nitrogen, and phosphorus-doped in the presence of an electric field.在电场存在下使用硼、氮和磷掺杂的[具体捕获对象未提及]的密度泛函理论研究。
Sci Rep. 2024 May 29;14(1):12388. doi: 10.1038/s41598-024-62301-x.
2
Substitution Models of Protein Evolution with Selection on Enzymatic Activity.蛋白质进化的替代模型与酶活性选择。
Mol Biol Evol. 2024 Feb 1;41(2). doi: 10.1093/molbev/msae026.
3
Picking the lock of coordination cage catalysis.破解配位笼催化的奥秘
Chem Sci. 2023 Sep 8;14(41):11300-11331. doi: 10.1039/d3sc02586a. eCollection 2023 Oct 25.
4
FeO@ABA-aniline-CuI nanocomposite as a highly efficient and reusable nanocatalyst for the synthesis of benzothiazole-sulfide aryls and heteroaryls.FeO@ABA-苯胺-碘化亚铜纳米复合材料作为一种高效且可重复使用的纳米催化剂用于合成苯并噻唑-硫化芳基和杂芳基。
RSC Adv. 2023 Jul 12;13(29):20351-20364. doi: 10.1039/d3ra03069e. eCollection 2023 Jun 29.
5
Kemp Eliminase Activity of Ketosteroid Isomerase.酮类固醇异构酶的肯普消除酶活性。
Biochemistry. 2017 Jan 31;56(4):582-591. doi: 10.1021/acs.biochem.6b00762. Epub 2017 Jan 20.
6
BIOPHYSICS. Response to Comments on "Extreme electric fields power catalysis in the active site of ketosteroid isomerase".生物物理学。对《极端电场助力酮甾类异构酶活性位点催化作用》评论的回应
Science. 2015 Aug 28;349(6251):936. doi: 10.1126/science.aab1627. Epub 2015 Aug 27.
本文引用的文献
1
Extreme electric fields power catalysis in the active site of ketosteroid isomerase.极强电场助力酮甾体异构酶活性位点的催化作用。
Science. 2014 Dec 19;346(6216):1510-4. doi: 10.1126/science.1259802.
2
Ground state destabilization from a positioned general base in the ketosteroid isomerase active site.定位在酮甾体异构酶活性部位的通用碱基使基态失稳。
Biochemistry. 2013 Feb 12;52(6):1074-81. doi: 10.1021/bi301348x. Epub 2013 Jan 30.
3
Dissecting the paradoxical effects of hydrogen bond mutations in the ketosteroid isomerase oxyanion hole.剖析酮甾体异构酶氧阴离子穴中氢键突变的矛盾效应。
Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):1960-5. doi: 10.1073/pnas.0911168107. Epub 2010 Jan 11.
4
Detection of large pKa perturbations of an inhibitor and a catalytic group at an enzyme active site, a mechanistic basis for catalytic power of many enzymes.
J Biol Chem. 2000 Dec 29;275(52):41100-6. doi: 10.1074/jbc.M007561200.
5
Solvation, reorganization energy, and biological catalysis.溶剂化、重组能与生物催化
J Biol Chem. 1998 Oct 9;273(41):26257-60. doi: 10.1074/jbc.273.41.26257.
Zotero 插件