Raudsepp-Hearne Ciara, Aiello Annette, Hussein Ahmed A, Heller Maria V, Johns Timothy, Capson Todd L
Smithsonian Tropical Research Institute, P.O. Box 0843-03092, Balboa, Ancón, Republic of Panama.
Department of Plant Science, McGill University, 21,111 Lakeshore Road, Ste. Anne de Bellevue, Quebec, Canada, H9X 3V9.
J Chem Ecol. 2015 Sep;41(9):816-21. doi: 10.1007/s10886-015-0614-6. Epub 2015 Aug 28.
We sought to compare the abilities of the specialist Lepidoptera Pyrrhopyge thericles (Hesperiidae) and the generalist Periphoba arcaei (Saturniidae) to assimilate three highly cytotoxic compounds from their larval host plant, Vismia baccifera (Clusiaceae) and to determine whether either insect discriminated in its assimilation of the compounds that are structurally similar but of variable cytotoxicity. Vismione B (1), deacetylvismione A (2), and deacetylvismione H (3) are cytotoxic compounds isolated from V. baccifera. Compound 1 was found in the 2nd and 3rd instars of P. arcaei, but not in the mature larvae or the pupae. Pyrrhopyge thericles assimilated trace quantities of compound 1 and deacetylvismione A (2), which were both found in the 3rd and 4th instars. In extracts of V. baccifera, compound 2 is present at levels approximately 6-fold greater than compound 1, indicating that the generalist P. arcaei is capable of selectively sequestering cytotoxic compounds from its host plant. Compounds 1 and 2 show comparable cytotoxicities in three different cancer cell lines, suggesting that properties other than cytotoxicity are responsible for the selective sequestration of 1 by P. arcaei. This study represents the first time that sequestration of this class of compounds has been recorded in the Lepidoptera.
我们试图比较专食性鳞翅目昆虫 Pyrrhopyge thericles（弄蝶科）和广食性昆虫 Periphoba arcaei（天蚕蛾科）从其幼虫寄主植物 Vismia baccifera（藤黄科）中同化三种高细胞毒性化合物的能力，并确定这两种昆虫在同化结构相似但细胞毒性不同的化合物时是否存在差异。Vismione B（1）、去乙酰基 Vismione A（2）和去乙酰基 Vismione H（3）是从 V. baccifera 中分离出的细胞毒性化合物。在 P. arcaei 的二龄和三龄幼虫中发现了化合物 1，但在成熟幼虫和蛹中未发现。Pyrrhopyge thericles 同化了痕量的化合物 1 和去乙酰基 Vismione A（2），这两种化合物在三龄和四龄幼虫中均有发现。在 V. baccifera 的提取物中，化合物 2 的含量约为化合物 1 的 6 倍，这表明广食性的 P. arcaei 能够从其寄主植物中选择性地隔离细胞毒性化合物。化合物 1 和 2 在三种不同的癌细胞系中表现出相当的细胞毒性，这表明除细胞毒性外的其他特性是 P. arcaei 选择性隔离化合物 1 的原因。这项研究首次记录了鳞翅目中这类化合物的隔离现象。
