University of Szeged, Faculty of Science and Informatics, Department of Microbiology, Szeged, Hungary.
University of Szeged, Faculty of Science and Informatics, Department of Microbiology, Szeged, Hungary Innsbruck Medical University, Biocenter, Division of Molecular Biology, Innsbruck, Austria
Med Mycol. 2015 Nov;53(8):890-5. doi: 10.1093/mmy/myv064. Epub 2015 Aug 26.
In the present study, in vitro antifungal activities of five antipsychotic drugs (i.e., chlorpromazine hydrochloride, CPZ; trifluoperazine hydrochloride, TPZ; amantadine hydrochloride; R-(-)-deprenyl hydrochloride, and valproic acid sodium salt) and five conventional antifungal drugs (i.e., amphotericin B, AMB; caspofungin, CSP; itraconazole; terbinafine, TRB and voriconazole, VRC) were investigated in broth microdilution tests against four clinical and five environmental Scedosporium and Pseudallescheria isolates. When used alone, phenothiazines CPZ and TPZ exerted remarkable antifungal effects. Thus, their in vitro combinations with AMB, CSP, VRC, and TRB were also examined against the clinical isolates. In combination with antifungal agents, CPZ was able to act synergistically with AMB and TRB in cases of one and two isolates, respectively. In all other cases, indifferent interactions were revealed. Antagonism was not observed between the tested agents. These combinations may establish a more effective and less toxic therapy after further in vitro and in vivo studies for Scedosporium and Pseudallescheria infections.
在本研究中，通过肉汤微量稀释试验检测了 5 种抗精神病药物（盐酸氯丙嗪、盐酸三氟拉嗪、盐酸金刚烷胺、R-(-)-deprenyl 盐酸盐和丙戊酸钠）和 5 种传统抗真菌药物（两性霉素 B、卡泊芬净、伊曲康唑、特比萘芬和伏立康唑）对 4 株临床和 5 株环境分离的枝孢菌属和拟青霉属的体外抗真菌活性。单独使用时，吩噻嗪类药物 CPZ 和 TPZ 表现出显著的抗真菌作用。因此，还研究了它们与 AMB、CSP、VRC 和 TRB 的体外联合应用对临床分离株的作用。与抗真菌药物联合使用时，CPZ 分别对 1 株和 2 株分离株与 AMB 和 TRB 表现出协同作用。在所有其他情况下，均显示出无关的相互作用。在测试的药物之间没有观察到拮抗作用。这些组合可能会在进一步的体外和体内研究后为枝孢菌属和拟青霉属感染建立更有效和更少毒性的治疗方法。
