Palermo Vanessa, Stirpe Mariarita, Torella Mirko, Falcone Claudio, Mazzoni Cristina
Pasteur Institute-Cenci Bolognetti Foundation, Department of Biology and Biotechnology 'Charles Darwin', Sapienza University of Rome, Piazzale Aldo Moro 5, 00185-Rome, Italy.
Pasteur Institute-Cenci Bolognetti Foundation, Department of Biology and Biotechnology 'Charles Darwin', Sapienza University of Rome, Piazzale Aldo Moro 5, 00185-Rome, Italy
FEMS Yeast Res. 2015 Nov;15(7). doi: 10.1093/femsyr/fov074. Epub 2015 Aug 26.
Saccharomyces cerevisiae mutants in the essential gene LSM4, involved in messenger RNA decapping, and expressing a truncated form of the LSM4 gene of the yeast Kluyveromyces lactis (Kllsm4Δ1), show premature aging accompanied by the presence of typical markers of apoptosis and high sensitivity to oxidative stressing agents. We isolated multicopy extragenic suppressors of these defects, transforming the Kllsm4Δ1 mutant with a yeast DNA library and selecting clones showing resistance to acetic acid. Here we present one of these clones, carrying a DNA fragment containing the NEM1 gene (Nuclear Envelope Morphology protein 1), which encodes the catalytic subunit of the Nem1p-Spo7p phosphatase holoenzyme. Nem1p regulates nuclear growth by controlling phospholipid biosynthesis and it is required for normal nuclear envelope morphology and sporulation. The data presented here correlate the mRNA metabolism with the biosynthesis of phospholipids and with the functionality of the endoplasmic reticulum.
酿酒酵母中参与信使核糖核酸去帽的必需基因LSM4的突变体，表达乳酸克鲁维酵母（Kluyveromyces lactis）LSM4基因的截短形式（Kllsm4Δ1），表现出早衰，伴有典型的凋亡标志物，并对氧化应激剂高度敏感。我们分离出了这些缺陷的多拷贝非基因抑制子，用酵母DNA文库转化Kllsm4Δ1突变体，并筛选出对乙酸有抗性的克隆。在此我们展示其中一个克隆，它携带一个包含NEM1基因（核膜形态蛋白1）的DNA片段，该基因编码Nem1p-Spo7p磷酸酶全酶的催化亚基。Nem1p通过控制磷脂生物合成来调节核生长，并且它是正常核膜形态和孢子形成所必需的。此处呈现的数据将信使核糖核酸代谢与磷脂生物合成以及内质网的功能联系起来。
