Chris Ajonijebu Duyilemi, Adeyemi Adeniyi Philip, Oloruntoba Adekeye Adeshina, Peter Olatunji Babawale, Olakunle Ishola Azeez, Michael Ogundele Olalekan
Department of Physiology, College of Medicine and Health Sciences, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria.
Department of Anatomy, Cell Biology and Neuroscience Unit, College of Medicine and Health Sciences, Afe Babalola University, College Building II, Room G14, Km 8.5 Afe Babalola Way, PMB 5454, Ado-Ekiti, Ekiti State, Nigeria.
J Neurodegener Dis. 2014;2014:359436. doi: 10.1155/2014/359436. Epub 2014 Nov 12.
In this study we evaluated the time dependence in cadmium-nicotine interaction and its effect on motor function, anxiety linked behavioural changes, serum electrolytes, and weight after acute and chronic treatment in adult male mice. Animals were separated randomly into four groups of n = 6 animals each. Treatment was done with nicotine, cadmium, or nicotine-cadmium for 21 days. A fourth group received normal saline for the same duration (control). Average weight was determined at 7-day interval for the acute (D1-D7) and chronic (D7-D21) treatment phases. Similarly, the behavioural tests for exploratory motor function (open field test) and anxiety were evaluated. Serum electrolytes were measured after the chronic phase. Nicotine, cadmium, and nicotine-cadmium treatments caused no significant change in body weight after the acute phase while cadmium-nicotine and cadmium caused a decline in weight after the chronic phase. This suggests the role of cadmium in the weight loss observed in tobacco smoke users. Both nicotine and cadmium raised serum Ca(2+) concentration and had no significant effect on K(+) ion when compared with the control. In addition, nicotine-cadmium treatment increased bioaccumulation of Cd(2+) in the serum which corresponded to a decrease in body weight, motor function, and an increase in anxiety.
在本研究中，我们评估了成年雄性小鼠在急性和慢性治疗后镉与尼古丁相互作用的时间依赖性及其对运动功能、焦虑相关行为变化、血清电解质和体重的影响。将动物随机分为四组，每组n = 6只动物。用尼古丁、镉或尼古丁 - 镉进行处理，持续21天。第四组在相同时间段内接受生理盐水（对照组）。在急性（第1天 - 第7天）和慢性（第7天 - 第21天）治疗阶段，每隔7天测定平均体重。同样，对探索性运动功能（旷场试验）和焦虑进行行为测试。在慢性阶段后测量血清电解质。急性阶段后，尼古丁、镉和尼古丁 - 镉处理对体重没有显著影响，而慢性阶段后，镉 - 尼古丁和镉导致体重下降。这表明镉在吸烟人群体重减轻中所起的作用。与对照组相比，尼古丁和镉均提高了血清Ca(2+)浓度，对K(+)离子没有显著影响。此外，尼古丁 - 镉处理增加了血清中Cd(2+)的生物蓄积，这与体重下降、运动功能降低以及焦虑增加相对应。
