Chon Jung-Whan, Yim Jin-Hyeok, Kim Hong-Seok, Kim Dong-Hyeon, Kim Hyunsook, Oh Deog-Hwan, Kim Soo-Ki, Seo Kun-Ho
1 Department of Animal Science and Technology, Konkuk University , Seoul, Korea.
2 KU Center for Food Safety, College of Veterinary Medicine, Konkuk University , Seoul, Korea.
Foodborne Pathog Dis. 2015 Sep;12(9):795-9. doi: 10.1089/fpd.2015.1977.
Ready-to-eat (RTE) foods such as prepared vegetables are becoming an increasingly popular food choice. Since RTE vegetables are not commonly sterilized by heat treatment, contamination with foodborne pathogens such as Bacillus cereus (B. cereus) is a major concern. The objective of this study was to assess the quantitative prevalence and toxin gene profiles of B. cereus strains isolated from RTE vegetables. We found that 70 of the 145 (48%) tested retail vegetable salad and sprout samples were positive for B. cereus. The B. cereus isolates harbored at least one enterotoxin gene. The detection rates of nheABC, hblCDA, cytK, and entFM enterotoxin genes among all isolates were 97.1%, 100%, 81.4%, and 98.6%, respectively. No strain carried the emetic toxin genes. Only 4 strains (5.7%) from the 70 isolates were psychrotrophic and were able to grow at 7°C. All of the psychrotrophic isolates possessed at least 1 enterotoxin gene.
即食（RTE）食品，如加工好的蔬菜，正成为越来越受欢迎的食物选择。由于即食蔬菜通常不通过热处理进行杀菌，因此受到食源性病原体如蜡样芽孢杆菌（B. cereus）污染是一个主要问题。本研究的目的是评估从即食蔬菜中分离出的蜡样芽孢杆菌菌株的定量流行情况和毒素基因谱。我们发现，在145份测试的零售蔬菜沙拉和豆芽样品中，有70份（48%）蜡样芽孢杆菌呈阳性。蜡样芽孢杆菌分离株至少携带一种肠毒素基因。在所有分离株中，nheABC、hblCDA、cytK和entFM肠毒素基因的检出率分别为97.1%、100%、81.4%和98.6%。没有菌株携带呕吐毒素基因。在70株分离株中，只有4株（5.7%）是嗜冷菌，能够在7°C下生长。所有嗜冷菌分离株都至少拥有1种肠毒素基因。
