使用微孔光密度计对斑点印迹进行定量放射自显影。
Quantitative autoradiography of dot blots using a microwell densitometer.
作者信息
Ross P M, Woodley K, Baird M
机构信息
Rockefeller University, New York, NY 10021.
出版信息
Biotechniques. 1989 Jul-Aug;7(7):680-8.
PMID:2631782
Abstract
We have established conditions for the quantitation of DNA hybridization by "reading" dot blot autoradiographs with a microwell plate densitometer. This method is more convenient, as accurate, and more sensitive than counting the spots in a liquid scintillation counter.
摘要
我们已经建立了通过用微孔板密度计“读取”斑点印迹放射自显影片来定量DNA杂交的条件。该方法比在液体闪烁计数器中计数斑点更方便、同样准确且更灵敏。
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