小球藻病毒XZ-6E腺嘌呤甲基转移酶M.CviRI编码基因的分子克隆与特性分析
Molecular cloning and characterization of the gene encoding the adenine methyltransferase M.CviRI from Chlorella virus XZ-6E.
作者信息
Stefan C, Xia Y N, Van Etten J L
机构信息
Department of Plant Pathology, University of Nebraska, Lincoln 68583-0722.
出版信息
Nucleic Acids Res. 1991 Jan 25;19(2):307-11. doi: 10.1093/nar/19.2.307.
The gene encoding the DNA methyltransferase M.CviRI from Chlorella virus XZ-6E was cloned and expressed in Escherichia coli. M.CviRI methylates adenine in TGCA sequences. DNA containing the M.CviRI gene was sequenced and a single open reading frame of 1137 bp was identified which could code for a polypeptide of 379 amino acids with a predicted molecular weight of 42,814. Comparison of the M.CviRI predicted amino acid sequence with another Chlorella virus and 14 bacterial adenine methyltransferases revealed extensive similarity to the other Chlorella virus enzyme.
从小球藻病毒XZ - 6E中编码DNA甲基转移酶M.CviRI的基因被克隆并在大肠杆菌中表达。M.CviRI使TGCA序列中的腺嘌呤甲基化。对含有M.CviRI基因的DNA进行测序，鉴定出一个1137 bp的单一开放阅读框，其可编码一个由379个氨基酸组成的多肽，预测分子量为42,814。将M.CviRI预测的氨基酸序列与另一种小球藻病毒和14种细菌腺嘌呤甲基转移酶进行比较，发现它与另一种小球藻病毒酶有广泛的相似性。
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