Arneodo Joel D, Balbi Emilia I, Flores Fernando M, Sciocco-Cap Alicia
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917, (1033) Buenos Aires, Argentina. Instituto de Microbiología y Zoología Agrícola (IMyZA) - Instituto Nacional de Tecnología Agropecuaria (INTA), Nicolás Repetto y de los Reseros, (1686) Hurlingham, Argentina.
EEA- INTA Marcos Juárez, Ruta 12 km 3, (2580) Marcos Juárez, Argentina.
J Econ Entomol. 2015 Dec;108(6):2505-10. doi: 10.1093/jee/tov254. Epub 2015 Aug 28.
Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae: Heliothinae) is among the most voracious global pests of agriculture. Adults of this species were identified recently in northern Argentina by dissection of male genitalia. In this work, a rapid and simple molecular tool was designed to distinguish H. armigera from the morphologically similar indigenous bollworms Helicoverpa zea (Boddie) and Helicoverpa gelotopoeon (Dyar), regardless of the life stage. Amplification of partial COI gene with a new primer pair, and subsequent digestion with endonuclease HinfI, yielded different RFLP profiles for the three main Helicoverpa pests currently present in South America. The method was validated in Helicoverpa specimens collected across Argentina, whose identity was further corroborated by COI sequencing and phylogenetic analysis. The data reported here constitute the first molecular confirmation of this pest in the country. The survey revealed the occurrence of H. armigera in northern and central Argentina, including the main soybean- and maize-producing area.
棉铃虫（Helicoverpa armigera (Hübner)，鳞翅目：夜蛾科：实夜蛾亚科）是全球最具暴食性的农业害虫之一。最近通过解剖雄性生殖器在阿根廷北部鉴定出了该物种的成虫。在这项研究中，设计了一种快速且简单的分子工具，用于区分棉铃虫与形态相似的本土棉铃虫，即美洲棉铃虫（Helicoverpa zea (Boddie)）和格氏棉铃虫（Helicoverpa gelotopoeon (Dyar)），无论处于何种生命阶段。用一对新引物扩增部分COI基因，随后用内切酶HinfI进行酶切，为目前南美洲存在的三种主要棉铃虫害虫产生了不同的限制性片段长度多态性（RFLP）图谱。该方法在从阿根廷各地收集的棉铃虫标本中得到验证，其身份通过COI测序和系统发育分析得到进一步证实。此处报告的数据构成了该国对这种害虫的首次分子确认。调查揭示了棉铃虫在阿根廷北部和中部的存在，包括主要的大豆和玉米产区。
