Wright Laura L, Turton Jane F, Hopkins Katie L, Livermore David M, Woodford Neil
Antimicrobial Resistance and Healthcare Associated Infections Reference Unit, Public Health England, 61 Colindale Avenue, London NW9 5EQ, UK Norwich Medical School, University of East Anglia, Norwich, Norfolk NR4 7TJ, UK
Antimicrobial Resistance and Healthcare Associated Infections Reference Unit, Public Health England, 61 Colindale Avenue, London NW9 5EQ, UK.
J Antimicrob Chemother. 2015 Dec;70(12):3250-8. doi: 10.1093/jac/dkv263. Epub 2015 Aug 27.
We sought to characterize the genetic environment of blaVIM and blaIMP genes in Pseudomonas aeruginosa isolates from the UK; these included members of six previously described prevalent complexes, A-F, which correspond to international 'high-risk clones', along with diverse strains.
Metallo-β-lactamase (MBL)-encoding class 1 integrons were amplified by PCR from 218 P. aeruginosa isolates producing VIM-type (n = 196) or IMP-type (n = 22) enzymes, referred from UK hospital laboratories between 2003 and 2012. The variable regions of selected integrons were sequenced using a primer walking method.
One-hundred-and-nineteen isolates had an MBL-encoding integron with the 3' conserved sequence (3'CS), 65 had Tn5090-like 3' regions and 17 had the sul1 gene, but lacked the qacEΔ1 gene; the 3' region could not be amplified using any primer combinations for the remaining 17 isolates. Six integron profiles were each seen in more than five isolates. Predominant integron types were seen amongst isolates belonging to STs 111, 233, 654/964 and 773 (complexes A, C, D and F, respectively), whereas diverse integron profiles were seen in isolates belonging to ST235 (complex B) and ST357 (complex E).
In UK P. aeruginosa isolates, MBL genes occur in diverse class 1 integron structures, though commonly with 3' regions containing the classical 3'CS or Tn5090-like regions. Four of the six main clonal complexes, referred from multiple laboratories, carried a predominant integron type, whereas the remaining two had more diverse types.
我们试图对来自英国的铜绿假单胞菌分离株中blaVIM和blaIMP基因的遗传环境进行表征；这些分离株包括先前描述的六个流行菌系（A - F）的成员，它们对应于国际“高风险克隆株”，以及多种菌株。
通过聚合酶链反应（PCR）从218株产生VIM型（n = 196）或IMP型（n = 22）酶的铜绿假单胞菌分离株中扩增出编码金属β - 内酰胺酶（MBL）的1类整合子，这些分离株于2003年至2012年间由英国医院实验室送检。使用引物步移法对选定整合子的可变区进行测序。
119株分离株具有编码MBL的整合子及3'保守序列（3'CS），65株具有Tn5090样3'区域，17株具有sul1基因，但缺少qacEΔ1基因；其余17株分离株使用任何引物组合均无法扩增出3'区域。六种整合子图谱在超过五株分离株中均有出现。在属于序列类型（ST）111、233、654/964和773的分离株（分别对应菌系A、C、D和F）中可见主要的整合子类型，而在属于ST235（菌系B）和ST357（菌系E）的分离株中可见多种整合子图谱。
在英国的铜绿假单胞菌分离株中，MBL基因存在于多种1类整合子结构中，尽管通常其3'区域包含经典的3'CS或Tn5090样区域。来自多个实验室的六个主要克隆菌系中的四个携带主要的整合子类型，而其余两个具有更多样化的类型。
