Kiraz Yağmur, Neergheen-Bhujun Vidushi S, Rummun Nawraj, Baran Yusuf
Department of Molecular Biology and Genetics, Izmir Institute of Technology, Urla, 35430, Izmir, Turkey.
Department of Molecular Biology and Genetics, Faculty of Life and Natural Sciences, Abdullah Gul University, Kayseri, Turkey.
Tumour Biol. 2016 Feb;37(2):1803-15. doi: 10.1007/s13277-015-3962-5. Epub 2015 Aug 29.
Multiple myeloma is of great concern since existing therapies are unable to cure this clinical condition. Alternative therapeutic approaches are mandatory, and the use of plant extracts is considered interesting. Punica granatum and its derived products were suggested as potential anticancer agents due to the presence of bioactive compounds. Thus, polypenolic-rich extracts of the non-edible parts of P. granatum were investigated for their antiproliferative and apoptotic effects on U266 multiple myeloma cells. We demonstrated that there were dose-dependent decreases in the proliferation of U266 cells in response to P. granatum extracts. Also, exposure to the extracts triggered apoptosis with significant increases in loss of mitochondrial membrane potential in U266 cells exposed to the leaves and stem extracts, while the flower extract resulted in slight increases in loss of MMP. These results were confirmed by Annexin-V analysis. These results documented the cytotoxic and apoptotic effects of P. granatum extracts on human U266 multiple myeloma cells via disruption of mitochondrial membrane potential and increasing cell cycle arrest. The data suggest that the extracts can be envisaged in cancer chemoprevention and call for further exploration into the potential application of these plant parts.
多发性骨髓瘤备受关注，因为现有疗法无法治愈这种临床病症。必须采用替代治疗方法，而使用植物提取物被认为是有意义的。由于含有生物活性化合物，石榴及其衍生产品被认为是潜在的抗癌剂。因此，对石榴不可食用部分富含多酚的提取物对U266多发性骨髓瘤细胞的抗增殖和凋亡作用进行了研究。我们证明，石榴提取物会使U266细胞的增殖呈剂量依赖性下降。此外，暴露于提取物会引发细胞凋亡，暴露于叶和茎提取物的U266细胞线粒体膜电位丧失显著增加，而花提取物导致线粒体膜电位丧失略有增加。这些结果通过膜联蛋白V分析得到证实。这些结果证明了石榴提取物通过破坏线粒体膜电位和增加细胞周期停滞对人U266多发性骨髓瘤细胞具有细胞毒性和凋亡作用。数据表明，这些提取物可用于癌症化学预防，并需要进一步探索这些植物部分的潜在应用。
