Lucci Paolo, Pacetti Deborah, Loizzo Monica R, Frega Natale G
Department of Nutrition and Biochemistry, Faculty of Sciences, Pontificia Universidad Javeriana, Bogotà D.C., Colombia.
Department of Agricultural, Food and Environmental Sciences, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
Food Chem. 2015 Jan 15;167:475-83. doi: 10.1016/j.foodchem.2014.06.123. Epub 2014 Jul 10.
This paper aims to provide a solid base for the utilisation of pomegranate whole seed ethanolic extract (PSEE) as a nutraceutical/functional food ingredient. PSEE was tested for its antioxidant and antiproliferative activities against different human cancer cell lines. Bioactive lipid compounds were identified by studying the PSEE lipid portion. PSEE exhibited a protection of lipid peroxidation threefold higher than a positive control. PSEE showed a promising antiproliferative activity against hormone dependent prostate carcinoma LNCaP, with an IC50 value 3 times lower than the positive control vinblastine, and against human breast cancer cell lines (IC50=9.6 μg/ml). PSEE contained lipid bioactive compounds, such as neutral lipids, consisting of 72.8% punicic acid, glycolipids and phospholipids rich in essential fatty acids (α-linoleic and α-linolenic acids). Due the presence of bioactive compounds and the remarkable antiproliferative activity, the use of PSEE as a value-added ingredient in formulations of products aimed to prevent diseases, especially cancer, could be promoted.
本文旨在为将石榴全籽乙醇提取物（PSEE）用作营养保健品/功能性食品成分提供坚实基础。对PSEE针对不同人类癌细胞系的抗氧化和抗增殖活性进行了测试。通过研究PSEE的脂质部分鉴定了生物活性脂质化合物。PSEE对脂质过氧化的保护作用比阳性对照高三倍。PSEE对激素依赖性前列腺癌LNCaP显示出有前景的抗增殖活性，其IC50值比阳性对照长春碱低3倍，对人乳腺癌细胞系也有抗增殖活性（IC50 = 9.6μg/ml）。PSEE含有脂质生物活性化合物，如中性脂质，其中石榴酸占72.8%，还有富含必需脂肪酸（α-亚油酸和α-亚麻酸）的糖脂和磷脂。由于存在生物活性化合物以及显著的抗增殖活性，可促进将PSEE用作旨在预防疾病尤其是癌症的产品配方中的增值成分。
