do Nascimento Adriana M, Soares Mateus Gonçalves, da Silva Torchelsen Fernanda K V, de Araujo Jorge A Viana, Lage Paula S, Duarte Mariana C, Andrade Pedro H R, Ribeiro Tatiana G, Coelho Eduardo A F, do Nascimento Andréa M
Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, CEP 14040-901, Ribeirão Prêto, SP, Brazil.
Departamento de Química, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Campus Universitário Morro do Cruzeiro, Bauxita, CEP 35400-000, Ouro Prêto, MG, Brazil.
World J Microbiol Biotechnol. 2015 Nov;31(11):1793-800. doi: 10.1007/s11274-015-1932-0. Epub 2015 Aug 29.
The purpose of this work was to evaluate the antileishmanial activity of endophytic fungi isolated from leaves of Vernonia polyanthes plant and their prospective use in the discovery of bioactive compounds. Sixteen endophytes were isolated by using potato dextrose agar medium and submitted to cultivation in rice medium. The fungal cultures were extracted with ethanol and used as crude extracts for testing their antileishmanial activity. The most active ethanol extract was obtained from P2-F3 strain, which was identified as Cochliobolus sativus by ITS rRNA gene sequence data. Followed by a bioassay-guided fractionation, the cochlioquinone A, isocochlioquinone A and anhydrocochlioquinone A compounds were isolated from the crude extracts and demonstrated to inhibit the parasites. From the present work, it is possible to conclude that endophytic fungi derived from medicinal plant V. polyanthes may be considered promising source for the discovery of bioactive compounds.
本研究旨在评估从多花斑鸠菊（Vernonia polyanthes）叶片中分离出的内生真菌的抗利什曼原虫活性，以及它们在发现生物活性化合物方面的潜在用途。使用马铃薯葡萄糖琼脂培养基分离出16株内生真菌，并将其接种于大米培养基中培养。用乙醇对真菌培养物进行提取，并将提取物作为粗提物用于测试其抗利什曼原虫活性。活性最强的乙醇提取物来自P2-F3菌株，通过ITS rRNA基因序列数据鉴定该菌株为禾旋孢腔菌（Cochliobolus sativus）。随后通过生物活性导向的分级分离，从粗提物中分离出了旋孢腔醌A、异旋孢腔醌A和脱水旋孢腔醌A化合物，并证明它们能抑制寄生虫。从目前的研究中可以得出结论，来源于药用植物多花斑鸠菊的内生真菌可能是发现生物活性化合物的有前途的来源。
