College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China.
World J Microbiol Biotechnol. 2012 May;28(5):2107-12. doi: 10.1007/s11274-012-1015-4. Epub 2012 Feb 12.
An endophytic fungus was isolated from the root of the medicinal plant Moringa oleifera Lam. Based on analyzing the rDNA sequence, the fungus was identified as Nigrospora sp. This is the first report of the isolation of endophytic Nigrospora from M. oleifera. By bioassay-guided fractionation, four antifungal secondary metabolites were isolated from liquid cultures of the fungus Nigrospora sp. LLGLM003, and their chemical structures were determined to be griseofulvin (1), dechlorogriseofulvin (2), 8-dihydroramulosin (3) and mellein (4) on the basis of spectroscopic analyses. Compound 2, 3 and 4 were isolated from Nigrospora sp. for the first time. In vitro antifungal assay showed that griseofulvin displayed clear inhibition of the growth of 8 plant pathogenic fungi. Dechlorogriseofulvin and mellein exhibited only weak antifungal activities, whereas 8-dihydroramulosin displayed no antifungal activities.
从药用植物辣木（Moringa oleifera Lam.）的根部分离出一种内生真菌。基于对 rDNA 序列的分析，该真菌被鉴定为黑曲霉（Nigrospora sp.）。这是首次从辣木中分离出内生黑曲霉。通过生物活性导向分离，从真菌 Nigrospora sp. LLGLM003 的液体培养物中分离出四种抗真菌次生代谢产物，并根据光谱分析确定它们的化学结构分别为灰黄霉素（1）、脱氯灰黄霉素（2）、8-二氢罗姆斯林（3）和密勒因（4）。化合物 2、3 和 4 是首次从黑曲霉属中分离得到的。体外抗真菌试验表明，灰黄霉素对 8 种植物病原真菌的生长有明显的抑制作用。脱氯灰黄霉素和密勒因仅表现出较弱的抗真菌活性，而 8-二氢罗姆斯林则没有抗真菌活性。
