Lu Lin, Ju Fang, Zhao Hui, Ma Xuezhen
Department of Oncology, The Second Affiliated Hospital of Qingdao Medical College, No. 127 South Siliu Road, Qingdao, 266042, Shandong, People's Republic of China.
Biotechnol Lett. 2015 Dec;37(12):2387-94. doi: 10.1007/s10529-015-1941-y. Epub 2015 Aug 29.
To investigate the role of microRNAs in doxorubicin resistance of breast cancer cells.
MicroRNA-134 was down-regulated in doxorubicin-resistant and doxorubicin-sensitive breast cancer samples and MCF-7/ADR cell lines. After transfection of miR-134 mimics, an MTT assay confirmed that the MCF-7/ADR cell proliferation was inhibited. In the presence of doxorubicin, there was inhibition of cell proliferation. Transfection of miR-134 mimics induced MCF-7/ADR cell apoptosis. The expression of ABCC1 was significantly upregulated in doxorubicin-resistant or -sensitive breast cancer tissues and MCF-7/ADR cell lines. Over-expression of miR-134 decreased the expression of ABCC1 at the protein level.
MicroRNA-134 modulates resistance to doxorubicin in breast cancer cells by downregulating the expression of ABCC1 which is known to encode the multidrug resistance-associated protein 1.
研究微小RNA在乳腺癌细胞阿霉素耐药中的作用。
微小RNA-134在阿霉素耐药和阿霉素敏感的乳腺癌样本及MCF-7/ADR细胞系中表达下调。转染miR-134模拟物后，MTT试验证实MCF-7/ADR细胞增殖受到抑制。在阿霉素存在的情况下，细胞增殖受到抑制。转染miR-134模拟物可诱导MCF-7/ADR细胞凋亡。ABCC1的表达在阿霉素耐药或敏感的乳腺癌组织及MCF-7/ADR细胞系中显著上调。miR-134的过表达在蛋白水平降低了ABCC1的表达。
微小RNA-134通过下调已知编码多药耐药相关蛋白1的ABCC1的表达来调节乳腺癌细胞对阿霉素的耐药性。
