Molecular Microbiology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee (IIT R), Roorkee, Uttarakhand 247667, India.
Molecular Microbiology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee (IIT R), Roorkee, Uttarakhand 247667, India.
Bioresour Technol. 2015 Dec;197:91-8. doi: 10.1016/j.biortech.2015.08.039. Epub 2015 Aug 22.
This study explored biodiesel production from a low cost, abundant, non-edible lignocellulosic biomass from aqueous extract of Cassia fistula L. (CAE) fruit pulp. The CAE was utilized as substrate for cultivating novel oleaginous yeast Rhodosporidium kratochvilovae HIMPA1. This oleaginous yeast accumulates high amount of triacylglycerides as large intracellular lipid droplets (4.35±0.54μm) using CAE as sole nutritional source. Total lipids (4.86±0.54g/l) with lipid content of 53.18% (w/w) were produced by R. kratochvilovae HIMPA1 on CAE. The FAME profile obtained revealed palmitic acid (C16:0) 43.06%, stearic acid (C18:0) 28.74%, and oleic acid (C18:1) 17.34% as major fatty acids. High saturated fatty acids content (72.58%) can be blended with high PUFA feedstocks to make it an industrially viable renewable energy product.
本研究探索了从低成本、丰富、非食用木质纤维素生物质——番泻叶（CAE）果浆的水提物中生产生物柴油。CAE 被用作新型产油酵母罗伦隐球酵母 HIMPA1 的培养底物。这种产油酵母利用 CAE 作为唯一的营养源，积累大量的三酰基甘油，形成大的细胞内脂滴（4.35±0.54μm）。罗伦隐球酵母 HIMPA1 在 CAE 上生产的总油脂（4.86±0.54g/l），其油脂含量为 53.18%（w/w）。获得的 FAME 图谱显示，主要脂肪酸为棕榈酸（C16:0）43.06%、硬脂酸（C18:0）28.74%和油酸（C18:1）17.34%。高饱和脂肪酸含量（72.58%）可与高 PUFA 原料混合，使其成为一种具有工业可行性的可再生能源产品。
