Deeba Farha, Kumar Kukkala Kiran, Rajacharya Girish H, Gaur Naseem A
International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi 110067, India.
J Fungi (Basel). 2021 Nov 13;7(11):967. doi: 10.3390/jof7110967.
Oleaginous yeast has great biotechnological potential and scientific interest, yet the molecular rationale of its cellular behavior to carbon and nitrogen ratios with concurrent lipid agglomeration remains elusive. Here, metabolomics adaptations of the in response to varying glucose and nitrogen concentrations have been investigated. In preliminary screening we found that 5% glucose (/) was optimal for further analysis in 3641. Hereafter, the effect of complementation to increase lipid agglomeration was evaluated with different nitrogen sources and their concentration. The results obtained illustrated that the biomass (13 g/L) and lipid (9.1 g/L) production were maximum on 5% (/) glucose and 0.12% (NH)SO. Furthermore, to shed lights on lipid accumulation induced by nitrogen-limitation, we performed metabolomic analysis of the oleaginous yeast 3641. Significant changes were observed in metabolite concentrations by qualitative metabolomics through gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS), which were mapped onto the governing metabolic pathways. Notable finding in this strain concerns glycerol and CDP-DAG metabolism wherein reduced production of glycerol and phospholipids induced a bypass leading to enhanced de-novo triacylglyceride synthesis. Collectively, our findings help in understanding the central carbon metabolism of which may assist in developing rationale metabolic models and engineering efforts in this organism.
产油酵母具有巨大的生物技术潜力和科研价值，然而其细胞行为对碳氮比及同时发生的脂质聚集的分子原理仍不清楚。在此，我们研究了产油酵母对不同葡萄糖和氮浓度的代谢组学适应性。在初步筛选中，我们发现5%葡萄糖（/）最适合在3641中进行进一步分析。此后，我们评估了不同氮源及其浓度对增加脂质聚集的补充作用。所得结果表明，在5%（/）葡萄糖和0.12%（NH）₂SO₄条件下，生物量（13 g/L）和脂质（9.1 g/L）产量最高。此外，为了阐明氮限制诱导的脂质积累，我们对产油酵母3641进行了代谢组学分析。通过气相色谱-质谱联用（GC-MS）和液相色谱-质谱联用（LC-MS）的定性代谢组学方法，观察到代谢物浓度发生了显著变化，并将其映射到主要代谢途径上。该菌株的显著发现涉及甘油和CDP-DAG代谢，其中甘油和磷脂产量的降低引发了一条旁路，导致从头合成三酰甘油增加。总的来说，我们的研究结果有助于理解产油酵母的中心碳代谢，这可能有助于建立该生物体的合理代谢模型并开展工程研究。
