Centre for Functional Ecology (CFE), Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456 Coimbra, Portugal.
Centre of Marine Sciences (CCMAR), University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
Environ Pollut. 2015 Nov;206:619-26. doi: 10.1016/j.envpol.2015.08.026. Epub 2015 Aug 27.
The present study aims at identifying, among six mammalian and fish cell lines, a sensitive cell line whose in vitro median inhibitory concentration (IC50) better matches the in vivo short-term Sparus aurata median lethal concentration (LC50). IC50s and LC50 were assessed after exposure to the widely used fungicide azoxystrobin (AZX). Statistical results were relevant for most cell lines after 48 h of AZX exposure, being H9c2 the most sensitive cells, as well as the ones which provided the best prediction of fish toxicity, with a LC50,96h/IC50,48h = 0.581. H9c2 cell proliferation upon 72 h of AZX exposure revealed a LC50,96h/IC50,72h = 0.998. Therefore, identical absolute sensitivities were attained for both in vitro and in vivo assays. To conclude, the H9c2 cell-based assay is reliable and represents a suitable ethical alternative to conventional fish assays for AZX, and could be used to get valuable insights into the toxic effects of other pesticides.
本研究旨在从六种哺乳动物和鱼类细胞系中鉴定出一种敏感细胞系，其体外半数抑制浓度（IC50）与体内短期褐牙鲷半数致死浓度（LC50）更好匹配。在用广泛使用的杀菌剂唑菌胺酯（AZX）进行暴露后，评估了 IC50 和 LC50。在 AZX 暴露 48 小时后，大多数细胞系的统计结果都是相关的，H9c2 是最敏感的细胞系，也是对鱼类毒性预测最好的细胞系，LC50,96h/IC50,48h=0.581。在 AZX 暴露 72 小时后，H9c2 细胞的增殖显示出 LC50,96h/IC50,72h=0.998。因此，两种体外和体内检测的绝对敏感性相同。总之，基于 H9c2 细胞的测定是可靠的，并且代表了一种替代常规鱼类测定用于 AZX 的合适的伦理方法，并且可以用于深入了解其他农药的毒性作用。
