Kianmehr Mojtaba, Amiri Mostafa, Ebrahimzadeh-Bideskan Alireza, Hajavi Jafar
1 Department of Medical Physics, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Islamic Republic of Iran.
2 Department of Basic Sciences, Faculty of Allied Medicine, Gonabad University of Medical Sciences, Gonabad, Islamic Republic of Iran.
Toxicol Ind Health. 2016 Nov;32(11):1902-1909. doi: 10.1177/0748233715597347. Epub 2016 Jul 10.
Exposure to chemicals is inevitable for certain occupational groups, particularly in industrial countries. Some cytogenetic tests such as comet assay are effectively utilized to screen genotoxic effects of these chemicals in humans. The purpose of this study has been to assess the DNA damage in construction painters' lymphocytes using the comet assay.
Fourteen male construction painters with 1 year of required work experience were randomly selected from the list of Painter Union in Gonabad city, Iran. In addition, 14 healthy male non-painters were randomly selected as controls. The DNA damage degree was determined using a fluorescence microscope and CometScore software. The obtained data were analyzed employing independent t-test using SPSS software v. 14 at the significant level of p < 0.05.
All participants were non-smokers and non-alcoholics. No significant differences were found between the two groups in terms of age, weight, height, body mass index, and systolic and diastolic blood pressure. The DNA damage in the painter group was significantly greater than that of the control group. Tail length (µm) was 12.63 ± 6.54 versus 2.90 ± 0.84, tail DNA (%) was 4.33 ± 1.84 versus 1.29 ± 0.43, and tail moment (µm) was 1.85 ± 0.98 versus 0.14 ± 0.20 in painter and control groups ( p < 0.001), respectively.
The chemicals used in oil paint may increase the DNA damage in painter blood lymphocytes.
对于某些职业群体来说，接触化学物质是不可避免的，尤其是在工业化国家。一些细胞遗传学检测方法，如彗星试验，被有效地用于筛查这些化学物质对人类的遗传毒性作用。本研究的目的是使用彗星试验评估建筑油漆工淋巴细胞中的DNA损伤情况。
从伊朗戈纳巴德市油漆工工会名单中随机挑选14名有1年所需工作经验的男性建筑油漆工。此外，随机挑选14名健康男性非油漆工作为对照。使用荧光显微镜和CometScore软件测定DNA损伤程度。使用SPSS软件v. 14在p < 0.05的显著水平下采用独立t检验对获得的数据进行分析。
所有参与者均不吸烟、不酗酒。两组在年龄、体重、身高、体重指数以及收缩压和舒张压方面均未发现显著差异。油漆工组的DNA损伤明显大于对照组。油漆工组和对照组的尾长（μm）分别为12.63 ± 6.54和2.90 ± 0.84，尾DNA（%）分别为4.33 ± 1.84和1.29 ± 0.43，尾矩（μm）分别为1.85 ± 0.98和0.14 ± 0.20（p < 0.001）。
油漆中使用的化学物质可能会增加油漆工血液淋巴细胞中的DNA损伤。
