Lungkaphin Anusorn, Pongchaidecha Anchalee, Palee Siripong, Arjinajarn Phatchawan, Pompimon Wilart, Chattipakorn Nipon
a Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
b Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Appl Physiol Nutr Metab. 2015 Oct;40(10):1031-7. doi: 10.1139/apnm-2015-0108. Epub 2015 Jun 17.
Oxidative stress plays an important role in the pathogenesis of ischemia/reperfusion (I/R) injury induced by cardiac dysfunction. Pinocembrin (5,7-dihydroxyflavanone) is a flavonoid found in propolis and in rhizomes of fingerroot (Boesenbergia pandurata) that is reported to have pharmacological properties including antimicrobial, antioxidant, and anti-inflammatory activities. The cardioprotective effects of pinocembrin in an I/R model were investigated in this study. Male Wistar rats (n = 20) were randomly divided into 2 groups to receive either pinocembrin (30 mg/kg body weight) or the vehicle intravenously. Thirty minutes later, the left anterior descending coronary artery of each rat was ligated for 30 min, and then reperfusion was allowed for 120 min. Cardiac function improved in the pinocembrin-treated group: the time to first ventricular fibrillation (VF) was significantly longer in the treated group (550 ± 54 s) than in the vehicle-only control group (330 ± 27 s) (p < 0.05). VF incidence and arrhythmia score were lower and infarcts were 49% smaller in the pinocembrin-treated group than in the control group (p < 0.05). In the pinocembrin-treated group, malondialdehyde levels and Bax/Bcl-2 ratios decreased, and the ratio of phosphorylated connexin 43 (phospho-Cx43) to total Cx43 increased in infarcted tissues compared with the non-infarcted area (p < 0.05). Pinocembrin exhibited cardioprotective effects during I/R, evidenced by improved cardiac function, fewer arrhythmias, and smaller infarcts in treated hearts than in controls. These benefits may be due to pinocembrin's antiapoptotic and anti-oxidative stress effects and its ability to increase the phosphorylation of Cx43 in ischemic myocardium.
氧化应激在心脏功能障碍引起的缺血/再灌注（I/R）损伤的发病机制中起重要作用。松属素（5,7 - 二羟基黄酮）是一种存在于蜂胶和莪术根茎中的黄酮类化合物，据报道具有抗菌、抗氧化和抗炎等药理特性。本研究探讨了松属素在I/R模型中的心脏保护作用。将20只雄性Wistar大鼠随机分为两组，分别静脉注射松属素（30 mg/kg体重）或赋形剂。30分钟后，结扎每只大鼠的左冠状动脉前降支30分钟，然后再灌注120分钟。松属素治疗组的心脏功能得到改善：治疗组首次出现心室颤动（VF）的时间（550±54秒）明显长于仅用赋形剂的对照组（330±27秒）（p<0.05）。松属素治疗组的VF发生率和心律失常评分较低，梗死面积比对照组小49%（p<0.05）。与未梗死区域相比，松属素治疗组梗死组织中的丙二醛水平和Bax/Bcl - 2比值降低，磷酸化连接蛋白43（磷酸化Cx43）与总Cx43的比值升高（p<0.05）。松属素在I/R期间表现出心脏保护作用，表现为治疗组心脏功能改善、心律失常减少、梗死面积小于对照组。这些益处可能归因于松属素的抗凋亡和抗氧化应激作用及其增加缺血心肌中Cx43磷酸化的能力。
