Takanosu Masamine, Kagawa Yumiko
Nasunogahara Animal Clinic, Ohtawara, Tochigi, Japan (Takanosu)North Lab, Shiroisi-ku, Sapporo, Hokkaido, Japan (Kagawa)
Nasunogahara Animal Clinic, Ohtawara, Tochigi, Japan (Takanosu)North Lab, Shiroisi-ku, Sapporo, Hokkaido, Japan (Kagawa).
J Vet Diagn Invest. 2015 Sep;27(5):645-50. doi: 10.1177/1040638715600197. Epub 2015 Aug 28.
Clonality testing based on polymerase chain reaction is an important tool for diagnosis of lymphoproliferative diseases. Many primers have been designed and used for canine clonality testing. Canine intestinal lymphoma is usually diagnosed pathologically by examination of excised intestinal or endoscopic biopsy tissues, and clonality testing is sometimes used to support the pathological diagnosis if this examination is inconclusive. In the present study, the sensitivity of each previously published primer set for clonality testing was examined by using formalin-fixed, paraffin-embedded sections from 39 cases pathologically diagnosed as canine intestinal lymphoma (large-cell type). All 39 cases were immunohistochemically positive for cluster of differentiation (CD)3. Thirty-two out of the 39 cases showed clonality in the T-cell receptor gamma (TRG) with at least 1 of the tested primers. The primer set with the highest sensitivity detected all 32 cases with TRG clonality, with a sensitivity of 82.1%. These results provide useful evidence for the selection of primer sets for clonality testing of canine intestinal lymphoma.
基于聚合酶链反应的克隆性检测是诊断淋巴增殖性疾病的重要工具。许多引物已被设计并用于犬类克隆性检测。犬肠道淋巴瘤通常通过检查切除的肠道组织或内镜活检组织进行病理诊断，如果该检查结果不明确，有时会使用克隆性检测来支持病理诊断。在本研究中，通过使用39例病理诊断为犬肠道淋巴瘤（大细胞型）的福尔马林固定、石蜡包埋切片，检测了先前发表的用于克隆性检测的每组引物的敏感性。所有39例病例的分化簇（CD）3免疫组化均呈阳性。39例病例中有32例在T细胞受体γ（TRG）中表现出克隆性，至少使用了1种测试引物。敏感性最高的引物组检测到了所有32例具有TRG克隆性的病例，敏感性为82.1%。这些结果为犬肠道淋巴瘤克隆性检测引物组的选择提供了有用的证据。
