Nasunogahara Animal Clinic, Ohtawara, Tochigi, Japan.
North Lab, Sapporo, Hokkaido, Japan.
J Vet Diagn Invest. 2022 Nov;34(6):972-976. doi: 10.1177/10406387221116285. Epub 2022 Aug 2.
Clonality assays based on antigen receptors are used as adjunct examinations in the diagnosis of lymphoproliferative diseases. We investigated the usefulness of the T-cell receptor beta () and T-cell receptor delta () loci in clonality assays for high-grade gastrointestinal (GI) lymphoma in dogs. For , we used primers reported previously; for , we designed primers for each of the and genes based on genomic sequences. Genomic DNA was extracted from 39 formalin-fixed, paraffin-embedded sections of high-grade GI lymphoma diagnosed histologically. The sensitivity of and primers for GI lymphoma was 41.0% and 38.5%, respectively, which was lower than the 82.1% sensitivity of T-cell receptor gamma () primers However, some cases that could not be detected using primers had clonality with either or primers. We found the locus to be more suitable as a first choice for the assay of canine lymphoma clonality than the and loci. However, the detection rate of T-cell clonality may be enhanced using and primers for lymphoma cases not detected using primers.
基于抗原受体的克隆性检测可作为辅助检查用于淋巴增殖性疾病的诊断。我们研究了 T 细胞受体 β（）和 T 细胞受体 δ（）基因座在犬高级胃肠道（GI）淋巴瘤克隆性检测中的作用。对于，我们使用了先前报道的引物；对于，我们根据基因组序列为每个和基因设计了引物。从 39 例经组织学诊断为高级 GI 淋巴瘤的福尔马林固定、石蜡包埋切片中提取基因组 DNA。和引物检测 GI 淋巴瘤的敏感性分别为 41.0%和 38.5%，低于 T 细胞受体 γ（）引物的 82.1%敏感性。然而，一些无法用引物检测到的病例具有或引物的克隆性。我们发现与和基因座相比，位点更适合作为犬淋巴瘤克隆性检测的首选。然而，对于使用引物未检测到的淋巴瘤病例，使用和引物可能会提高 T 细胞克隆性的检测率。
