Reichert Stefan, Schlumberger Wolfgang, Dähnrich Cornelia, Hornig Nora, Altermann Wolfgang, Schaller Hans-Günter, Schulz Susanne
Department of Operative Dentistry and Periodontology, Martin-Luther University Halle-Wittenberg, Große Steinstrasse 19, 06108, Halle (Saale), Germany.
EUROIMMUN AG, Lubeck, Germany.
J Transl Med. 2015 Aug 29;13:283. doi: 10.1186/s12967-015-0625-7.
Periodontal disease could be a risk factor for rheumatoid arthritis (RA). It is assumed that the bacterial strain Porphyromonas gingivalis mediates citrullination of host peptides and thereby the generation of RA-associated autoantibodies in genetically predisposed individuals. For that reason non-RA individuals who suffered from generalized aggressive (GAgP, N = 51) and generalized chronic periodontitis (GChP, N = 50) were investigated regarding the occurrence of antibodies against citrullinated cyclic peptides (anti-CCP) and citrullinated α-enolase peptide-1 (anti-CEP-1) in comparison to non-RA non-periodontitis controls (N = 89). Furthermore, putative associations between infections with five periodontopathic bacteria or expression of certain human leucocyte antigens (HLA) to these autoantibodies were investigated.
The presence of anti-CCP and anti-CEP-1 in plasma samples was conducted with enzyme linked immunosorbent assay. Subgingival plaque specimens were taken from the deepest pocket of each quadrant and pooled. For detection of DNA of five periodontopathic bacteria PCR with sequence specific oligonucleotides was carried out. Low resolution HLA typing was carried out with PCR with sequence specific primers. Differences between patients and controls were assessed using Chi square test with Yates correction or Fisher`s exact test if the expected number n in one group was <5.
Two patients with GAgP (3.9%), no patient with GChP and two controls (2.2%, pFisher = 0.662) were positive for anti-CEP-1 whereas no study participant was anti-CCP positive. Individuals with P. gingivalis were slightly more often anti-CEP-1 positive in comparison to individuals without P. gingivalis (3.2 vs. 1.1%, pFisher = 0.366). Carrier of HLA-DQB106 or the HLA combination DRB113; DRB3*; DQB1*06 were slightly more anti-CEP-1 positive (6.1 and 4.3%) than no carriers (0.7 and 0%, pFisher 0.053).
GAgP and GChP and the presence of periodontopathic bacteria are not associated with an increased risk for occurrence of anti-CCP and anti-CEP-1 autoantibodies. The putative relationship between periodontitis and RA should be investigated in further studies.
牙周病可能是类风湿性关节炎（RA）的一个风险因素。据推测，牙龈卟啉单胞菌菌株介导宿主肽的瓜氨酸化，从而在遗传易感个体中产生与RA相关的自身抗体。因此，对患有广泛侵袭性牙周炎（GAgP，N = 51）和广泛慢性牙周炎（GChP，N = 50）的非RA个体，与非RA非牙周炎对照者（N = 89）相比，就抗瓜氨酸化环肽抗体（抗CCP）和抗瓜氨酸化α -烯醇酶肽 - 1抗体（抗CEP - 1）的出现情况进行了调查。此外，还研究了五种牙周病原菌感染或某些人类白细胞抗原（HLA）的表达与这些自身抗体之间的假定关联。
采用酶联免疫吸附测定法检测血浆样本中抗CCP和抗CEP - 1的存在情况。从每个象限最深的牙周袋中采集龈下菌斑标本并合并。采用序列特异性寡核苷酸的聚合酶链反应（PCR）检测五种牙周病原菌的DNA。采用序列特异性引物的PCR进行低分辨率HLA分型。如果一组中的预期数n <5，则使用校正的卡方检验或Fisher精确检验评估患者与对照之间的差异。
两名GAgP患者（3.9%）、无GChP患者以及两名对照者（2.2%，pFisher = 0.662）抗CEP - 1呈阳性，而没有研究参与者抗CCP呈阳性。与没有牙龈卟啉单胞菌的个体相比，感染牙龈卟啉单胞菌的个体抗CEP - 1阳性的比例略高（3.2%对1.1%，pFisher = 0.366）。携带HLA - DQB106或HLA组合DRB113；DRB3*；DQB1*06的个体抗CEP - 1阳性的比例（6.1%和4.3%）略高于非携带者（0.7%和0%，pFisher 0.053）。
GAgP和GChP以及牙周病原菌的存在与抗CCP和抗CEP - 1自身抗体出现风险增加无关。牙周炎与RA之间的假定关系应在进一步研究中进行调查。
