Sotnik Natalia V, Azizova Tamara V, Darroudi Firouz, Ainsbury Elizabeth A, Moquet Jayne E, Fomina Janna, Lloyd David C, Hone Pat A, Edwards Alan A
Southern Urals Biophysics Institute (SUBI), 19 Ozyorskoe Shosse, Ozyorsk, Chelyabinsk Region, 456780, Russian Federation.
Department of Toxicogenetics, Leiden University Medical Center, Leiden, The Netherlands.
Radiat Environ Biophys. 2015 Nov;54(4):445-51. doi: 10.1007/s00411-015-0614-5. Epub 2015 Aug 30.
The aim of this study was to apply the fluorescence in situ hybridization (FISH) translocation assay in combination with chromosome painting of peripheral blood lymphocytes for retrospective biological dosimetry of Mayak nuclear power plant workers exposed chronically to external gamma radiation. These data were compared with physical dose estimates based on monitoring with badge dosimeters throughout each person's working life. Chromosome translocation yields for 94 workers of the Mayak production association were measured in three laboratories: Southern Urals Biophysics Institute, Leiden University Medical Center and the former Health Protection Agency of the UK (hereinafter Public Health England). The results of the study demonstrated that the FISH-based translocation assay in workers with prolonged (chronic) occupational gamma-ray exposure was a reliable biological dosimeter even many years after radiation exposure. Cytogenetic estimates of red bone marrow doses from external gamma rays were reasonably consistent with dose measurements based on film badge readings successfully validated in dosimetry system "Doses-2005" by FISH, within the bounds of the associated uncertainties.
本研究的目的是将荧光原位杂交（FISH）易位分析与外周血淋巴细胞染色体涂染相结合，用于对长期暴露于外部γ辐射的玛雅克核电站工人进行回顾性生物剂量测定。将这些数据与基于每个人整个工作生涯中使用徽章剂量计监测得出的物理剂量估计值进行比较。在三个实验室对玛雅克生产协会的94名工人的染色体易位率进行了测量：南乌拉尔生物物理研究所、莱顿大学医学中心和英国前卫生防护局（以下简称英国公共卫生部）。研究结果表明，对于长期（慢性）职业性γ射线暴露的工人，基于FISH的易位分析即使在辐射暴露多年后仍是一种可靠的生物剂量计。在相关不确定性范围内，通过FISH在“Doses - 2005”剂量测定系统中成功验证的基于胶片徽章读数的外部γ射线对红骨髓剂量的细胞遗传学估计与剂量测量结果合理一致。
