Department of Chemical and Biomolecular Engineering, Carl R Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL 61801, United States.
Department of Chemical and Biomolecular Engineering, Carl R Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL 61801, United States; Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue , Urbana, IL 61801, United States; Department of Biochemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue , Urbana, IL 61801, United States; Department of Bioengineering, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue , Urbana, IL 61801, United States.
Curr Opin Biotechnol. 2015 Dec;36:85-90. doi: 10.1016/j.copbio.2015.08.003. Epub 2015 Aug 28.
Regulatory RNAs are increasingly recognized and utilized as key modulators of gene expression in diverse organisms. Thanks to their modular and programmable nature, trans-acting regulatory RNAs are especially attractive in genome-scale applications. Here we discuss the recent examples in microbial genome engineering implementing various trans-acting RNA platforms, including sRNA, RNAi, asRNA and CRISRP-Cas. In particular, we focus on how the scalable and multiplex nature of trans-acting RNAs has been used to tackle the challenges in creating genome-wide and combinatorial diversity for functional genomics and metabolic engineering applications. Advances in computational design and context-dependent regulation are also discussed for their contribution in improving fine-tuning capabilities of trans-acting RNAs.
调控 RNA 作为不同生物中基因表达的关键调节剂，正日益受到重视和应用。由于它们具有模块化和可编程的特性，反式作用调控 RNA 在基因组规模的应用中特别有吸引力。在这里，我们讨论了在微生物基因组工程中实施各种反式作用 RNA 平台的最新例子，包括 sRNA、RNAi、asRNA 和 CRISPR-Cas。特别是，我们专注于反式作用 RNA 的可扩展性和多重性如何被用于解决在功能基因组学和代谢工程应用中创建全基因组和组合多样性的挑战。我们还讨论了计算设计和上下文相关调节的进展，因为它们有助于提高反式作用 RNA 的微调能力。
