Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People's Republic of China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214000, People's Republic of China.
J Ind Microbiol Biotechnol. 2018 Oct;45(10):869-880. doi: 10.1007/s10295-018-2057-x. Epub 2018 Aug 3.
Saccharomyces cerevisiae strains with favorable characteristics are preferred for application in industries. However, the current ability to reprogram a yeast cell on the genome scale is limited due to the complexity of yeast ploids. In this study, a method named genome replication engineering-assisted continuous evolution (GREACE) was proved efficient in engineering S. cerevisiae with different ploids. Through iterative cycles of culture coupled with selection, GREACE could continuously improve the target traits of yeast by accumulating beneficial genetic modification in genome. The application of GREACE greatly improved the tolerance of yeast against acetic acid compared with their parent strain. This method could also be employed to improve yeast aroma profile and the phenotype could be stably inherited to the offspring. Therefore, GREACE method was efficient in S. cerevisiae engineering and it could be further used to evolve yeast with other specific characteristics.
具有优良特性的酿酒酵母菌株更适合应用于工业领域。然而，由于酵母倍性的复杂性，目前在基因组范围内对酵母细胞进行重编程的能力有限。在这项研究中，一种名为基因组复制工程辅助连续进化（GREACE）的方法被证明在工程不同倍性的酿酒酵母方面非常有效。通过与选择相结合的培养迭代循环，GREACE 可以通过在基因组中积累有益的遗传修饰来持续改善酵母的目标特性。与亲本菌株相比，GREACE 的应用大大提高了酵母对乙酸的耐受性。该方法还可用于改善酵母的香气特征，表型可以稳定遗传给后代。因此，GREACE 方法在酿酒酵母工程中非常有效，它可以进一步用于进化具有其他特定特性的酵母。
