El-Kenawy Ayman El-Meghawry, Elshama Said Said, Osman Hosam-Eldin Hussein
Department of molecular biology, GEBRI, University of Sadat, Egypt E-mail :
Asian Pac J Cancer Prev. 2015;16(14):5863-8. doi: 10.7314/apjcp.2015.16.14.5863.
Nicotine-derived nitrosamine ketone (NNK) is considered a key tobacco smoke carcinogen inducing lung tumors. Physalis peruviana L (harankash) is considered one plant with marked health benefits. This study aimed to evaluate Physalis peruviana L effect on the toxic effect of NNK induced lung cancer in the rats by using pulmonary histopathological, immunohistochemical and DNA flow cytometric analyses. Sixty adult male rats were divided into four groups, each consisting of fifteen animals. The first group received saline, the second received two successive toxic doses of NNK only while the third received two successive toxic doses of NNK with a single daily dose of Physalis peruviana L. The fourth group received a single daily dose of Physalis peruviana L only. Toxic doses of NNK induced hyperplasia and adenocarcinoma in the lung and positive immunoreactivity for Ki-67 and p53 staining with disturbance of the lung DNA content. Administration of Physalis peruviana L with NNK led to a mild pulmonary hyperplasia and weak expression of Ki-67 and p53 with an improvement in the lung DNA content. Physalis peruviana L may protect against NNK induced lung carcinogenesis due to its antioxidant and anti-proliferative effects.
尼古丁衍生的亚硝胺酮（NNK）被认为是导致肺癌的关键烟草烟雾致癌物。灯笼果被认为是一种具有显著健康益处的植物。本研究旨在通过肺组织病理学、免疫组织化学和DNA流式细胞术分析，评估灯笼果对NNK诱导的大鼠肺癌毒性作用的影响。60只成年雄性大鼠被分为四组，每组15只。第一组接受生理盐水，第二组仅接受连续两次NNK毒性剂量，第三组接受连续两次NNK毒性剂量并每日单次给予灯笼果，第四组仅每日单次给予灯笼果。NNK毒性剂量诱导肺部增生和腺癌，Ki-67和p53染色呈阳性免疫反应，肺DNA含量紊乱。NNK与灯笼果一起给药导致轻度肺增生，Ki-67和p53表达减弱，肺DNA含量得到改善。由于其抗氧化和抗增殖作用，灯笼果可能预防NNK诱导的肺癌发生。
