Humenik Martin, Smith Andrew M, Arndt Sina, Scheibel Thomas
Biomaterials, Faculty of Engineering Science, Universität Bayreuth, Universitätsstraße 30, 95440 Bayreuth, Germany.
Biomaterials, Faculty of Engineering Science, Universität Bayreuth, Universitätsstraße 30, 95440 Bayreuth, Germany ; Bayreuth Center for Colloids and Interfaces (BZKG), Universität Bayreuth, Universitätsstraße 30, 95440 Bayreuth, Germany ; Research Center Bio-Macromolecules (BIOmac), Universität Bayreuth, Universitätsstraße 30, 95440 Bayreuth, Germany ; Bayreuth Center for Molecular Biosciences (BZMB), Universität Bayreuth, Universitätsstraße 30, 95440 Bayreuth, Germany ; Bayreuth Center for Material Science (BayMAT), Universität Bayreuth, Universitätsstraße 30, 95440 Bayreuth, Germany.
Data Brief. 2015 Jul 29;4:571-6. doi: 10.1016/j.dib.2015.07.023. eCollection 2015 Sep.
This data article includes size exclusion chromatography data of soluble eADF4(C16), an engineered spider silk variant based on the core domain sequence of the natural dragline silk protein ADF4 of Araneus diadematus, in combination with light scattering; the protein is monomeric before assembly. The assembled mature fibrils were visualized by transmission electron microscopy (TEM) and atomic force microscopy (AFM). Sonicated fibrils were used as seeds to by-pass the nucleation lag phase in eADF4(C16) assembly. We also provide data on the sedimentation kinetics of spider silk in the presence of different NaCl concentrations revealing very slow protein aggregation in comparison to the fast assembly triggered by phosphate ions published previously [1]. Experiments in the Data article represent supporting material for our work published recently [1], which described the assembly mechanism of recombinant eADF4(C16) fibrils.
本数据文章包含可溶性eADF4(C16)的尺寸排阻色谱数据，eADF4(C16)是一种基于十字园蛛天然拖牵丝蛋白ADF4核心结构域序列构建的蜘蛛丝变体，并结合了光散射；该蛋白在组装前为单体。通过透射电子显微镜（TEM）和原子力显微镜（AFM）观察组装后的成熟纤维。超声处理的纤维用作种子，以绕过eADF4(C16)组装中的成核延迟阶段。我们还提供了在不同NaCl浓度下蜘蛛丝沉降动力学的数据，揭示了与先前发表的由磷酸根离子触发的快速组装相比非常缓慢的蛋白质聚集[1]。本数据文章中的实验为我们最近发表的工作[1]提供了支持材料，该工作描述了重组eADF4(C16)纤维的组装机制。
