基于抗微生物工程重组蜘蛛丝蛋白的二维和三维材料的数据。
Data for microbe resistant engineered recombinant spider silk protein based 2D and 3D materials.
作者信息
Kumari Sushma, Lang Gregor, DeSimone Elise, Spengler Christian, Trossmann Vanessa T, Lücker Susanne, Hudel Martina, Jacobs Karin, Krämer Norbert, Scheibel Thomas
机构信息
Department of Biomaterials, Faculty of Engineering Science, University of Bayreuth, Prof.-Rüdiger-Bormann-Str. 1, 95447 Bayreuth, Germany.
Biopolymer Processing Group, Faculty of Engineering Science, University of Bayreuth, Ludwig-Thoma-Str. 36A, 95447 Bayreuth, Germany.
出版信息
Data Brief. 2020 Sep 11;32:106305. doi: 10.1016/j.dib.2020.106305. eCollection 2020 Oct.
Data presented in this article describe bacterial and fungal repellent properties of 2D-films and 3D-hydrogels made of different recombinantly produced spider silk proteins based on consensus sequences of Araneus diadematus dragline silk proteins (fibroin 3 and 4). Here, the attachment, growth, and microbial colonization of Streptococcus mutans (S. mutans) as well as Candida albicans (C. albicans) on plane and micro-patterned films were visualized by scanning electron microscopy (SEM). Also, microbial viability data are provided of Escherichia coli (E. coli) and Pichia pastoris (P. pastoris) on hydrogels made of eADF4(C16) and eADF4(C16)-RGD, quantified using the Alamar blue assay. Experimental results, design of a post-operative contamination model of microbes with mammalian cells, and methods in the data article refer to the research paper "Engineered spider silk-based 2D and 3D materials prevent microbial infestation" published recently [1].
本文所呈现的数据描述了基于园蛛拖牵丝蛋白（丝心蛋白3和4）共有序列，由不同重组生产的蜘蛛丝蛋白制成的二维薄膜和三维水凝胶的细菌和真菌驱避特性。在此，通过扫描电子显微镜（SEM）观察了变形链球菌（S. mutans）以及白色念珠菌（C. albicans）在平面和微图案化薄膜上的附着、生长和微生物定植情况。此外，还提供了大肠杆菌（E. coli）和毕赤酵母（P. pastoris）在由eADF4(C16)和eADF4(C16)-RGD制成的水凝胶上的微生物活力数据，使用阿拉玛蓝测定法进行定量。实验结果、哺乳动物细胞微生物术后污染模型的设计以及数据文章中的方法参考了最近发表的研究论文《工程化蜘蛛丝基二维和三维材料预防微生物感染》[1]。
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