Xu Xiaoyu, Li Ning, Zhu Lin, Zhou Yuan, Cheng Huilin
a Department of Neurosurgery , Jinling Hospital, School of Medicine, Nanjing University , Nanjing , Jiangsu Province , China.
J Spinal Cord Med. 2016;39(2):220-8. doi: 10.1179/2045772315Y.0000000051. Epub 2015 Aug 31.
The primary focus of this study was to investigate the effects of local profound hypothermia and to explore the possible mechanism in adult rats with spinal cord injury.
Spinal cord injury models were established by placing aneurysm clips on T10. An epidural perfusion device was applied to maintain a steady temperature (18 °C) for 120 min with gradual rewarming to 37 °C Total hypothermic duration lasted up to about 170 min. The expression of axon regeneration inhibitors was tested by Western blot and real-time PCR. Luxol Fast Blue (LFB) stain and Bielschowsky silver stain were used to observe spinal cord morphology. Motor function of the hind limbs (BBB score) was monitored for 21 days.
The expressions of RhoA, ROCK-II, NG2, Neurocan, Brevican, and Nogo-A were downregulated by regional hypothermia (RH) after spinal cord injury. Subsequent observation showed that rats that had received RH had an alleviated demyelinating condition and a greater number of nerve fibers. Furthermore, the RH group achieved higher BBB scores than the spinal cord injury (SCI) group.
Recovery of hind limb function in rats can be promoted by local profound hypothermia; this may be caused by the suppression of axon regeneration inhibitors.
本研究的主要重点是研究局部深度低温的影响，并探讨成年大鼠脊髓损伤的可能机制。
通过在T10放置动脉瘤夹建立脊髓损伤模型。应用硬膜外灌注装置将温度维持在稳定的18°C达120分钟，然后逐渐复温至37°C。总低温持续时间长达约170分钟。通过蛋白质免疫印迹法和实时聚合酶链反应检测轴突再生抑制剂的表达。采用Luxol Fast Blue（LFB）染色和Bielschowsky银染色观察脊髓形态。对后肢运动功能（BBB评分）进行21天监测。
脊髓损伤后，局部低温（RH）使RhoA、ROCK-II、NG2、Neurocan、Brevican和Nogo-A的表达下调。随后的观察表明，接受RH治疗的大鼠脱髓鞘状况得到缓解，神经纤维数量更多。此外，RH组的BBB评分高于脊髓损伤（SCI）组。
局部深度低温可促进大鼠后肢功能恢复；这可能是由于轴突再生抑制剂受到抑制所致。
