Molecular Biology Institute of Barcelona (IBMB), Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain.
Department of Microbiology and Immunology, School of Pharmacy, Zagazig University, Zagazig, Egypt.
Nat Methods. 2015 Oct;12(10):955-8. doi: 10.1038/nmeth.3556. Epub 2015 Aug 31.
Protein tagging is widely used in approaches ranging from affinity purification to fluorescence-based detection in live cells. However, an intrinsic limitation of tagging is that the native function of the protein may be compromised or even abolished by the presence of the tag. Here we describe and characterize a set of small, innocuous protein tags (inntags) that we anticipate will find application in a variety of biological techniques.
蛋白质标记广泛应用于从亲和纯化到活细胞荧光检测等方法中。然而，标记的一个固有局限性是，标记的存在可能会损害甚至破坏蛋白质的天然功能。在这里，我们描述并表征了一组小的、无害的蛋白质标记（inntags），我们预计它们将在各种生物技术中得到应用。
