Tsutsumi Y, Kakumu S, Yoshioka K, Arao M, Inoue M, Wakita T
Third Department of Medicine, Nagoya University School of Medicine, Japan.
Digestion. 1989;44(4):191-9. doi: 10.1159/000199911.
Collagen formation is an important function of liver parenchymal and nonparenchymal cells that may be relevant to the pathogenesis of hepatic fibrosis. In the present study, the effect of various kinds of cytokines and prednisolone on collagen synthesis by hepatocytes obtained from perfused rat livers and confluent human skin fibroblasts were investigated. The cells were cultured in serum-free medium for 24 h with [3H]proline and various additives. The amounts of collagen and noncollagen protein synthesis were calculated from the incorporation of [3H]proline into collagen-sensitive and collagen-resistant proteins. The additions of interleukin 1 alpha, interleukin 1 beta and transforming growth factor alpha enhanced both collagen and noncollagen protein synthesis by hepatocytes as well as by fibroblasts. Transforming growth factor beta also increased both collagen and noncollagen protein synthesis by fibroblasts while it selectively inhibited collagen synthesis by hepatocytes. Interferon preparations and prednisolone showed a selective suppression of collagen synthesis by both cells, indicating that these additives did not affect either cell viability or proliferation during the observed period. These results suggest that collagen synthesis by liver parenchymal cells is either positively or negatively modulated by various kinds of cytokines and hormone.
胶原蛋白的形成是肝实质细胞和非实质细胞的一项重要功能，这可能与肝纤维化的发病机制相关。在本研究中，研究了各种细胞因子和泼尼松龙对从灌注大鼠肝脏获得的肝细胞以及汇合的人皮肤成纤维细胞胶原蛋白合成的影响。将细胞在无血清培养基中与[3H]脯氨酸及各种添加剂一起培养24小时。根据[3H]脯氨酸掺入对胶原蛋白敏感和抗胶原蛋白的蛋白质中情况，计算胶原蛋白和非胶原蛋白的合成量。添加白细胞介素1α、白细胞介素1β和转化生长因子α可增强肝细胞和成纤维细胞的胶原蛋白和非胶原蛋白合成。转化生长因子β也增加成纤维细胞的胶原蛋白和非胶原蛋白合成，而它选择性抑制肝细胞的胶原蛋白合成。干扰素制剂和泼尼松龙对两种细胞的胶原蛋白合成均有选择性抑制作用，表明在观察期内这些添加剂不影响细胞活力或增殖。这些结果提示，肝实质细胞的胶原蛋白合成受到各种细胞因子和激素的正向或负向调节。
