高效液相色谱法同时测定乌药中三种倍半萜内酯的含量

[Simultaneous determination of three sesquiterpene lactones in Radix Linderae by HPLC].

作者信息

Zheng Yunliang, Luan Lianjun, Gan Lishe, Zhou Changxin, Wu Yongjiang

机构信息

Institute of Modern Chinese Medicine, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2009 Nov;34(21):2777-80.

PMID:20209914

Abstract

OBJECTIVE

To develop an HPLC method for simultaneous determination of three major sesquiterpene lactones in Radix Linderae.

METHOD

The chromatographic separation was achieved on a Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) using isocratic elution of acetonitrile-water (containing 0.1% H3 PO4) (45 : 55) at a flow rate of 1.0 mL x min(-1). Detection was carried out using a photodiode array detector at 220 nm.

RESULT

The calibration curves were linear in the range of 0.001 8-0.036 0 g x L(-1) for hydroxylinderstrenolide (R2 = 0.999 8), 0.016 2-0.323 2 g x L(-1) for neolinderalactone (R2 = 0.999 9), 0.010 5-0.209 9 g x L(-1) for linderane (R2 = 0.999 9), respectively. The average recoveries were 100.0% for hydroxylinderstrenolide, 98.8% for neolinderalactone and 98.9% for linderane with RSD not more than 3.3%.

CONCLUSION

The established method was proved to be simple, sensitive and credible, and can be applied to quality control of Radix Linderae.

摘要

目的

建立高效液相色谱法同时测定乌药中3种主要倍半萜内酯的含量。

方法

采用Diamonsil C18柱（4.6 mm×250 mm，5μm），以乙腈-水（含0.1% H3PO4）（45∶55）为流动相，等度洗脱，流速为1.0 mL·min-1，用二极管阵列检测器在220 nm波长处检测。

结果

羟基络石内酯在0.001 8～0.036 0 g·L-1范围内线性关系良好（R2 = 0.999 8），新乌药内酯在0.016 2～0.323 2 g·L-1范围内线性关系良好（R2 = 0.999 9），乌药醚内酯在0.010 5～0.209 9 g·L-1范围内线性关系良好（R2 = 0.999 9）。羟基络石内酯、新乌药内酯和乌药醚内酯的平均回收率分别为100.0%、98.8%、98.9%，RSD均不超过3.3%。