Biotechnological Processes Unit, Móstoles (Madrid), Spain.
Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, Valladolid University, Valladolid, Spain.
Bioengineered. 2023 Dec;14(1):2286723. doi: 10.1080/21655979.2023.2286723. Epub 2023 Nov 27.
can accumulate microbial oils using short-chain fatty acids (SCFA) as carbon sources. SCFAs-rich media often contain significant amounts of nitrogen that prevent high carbon:nitrogen (C:N) ratios necessary to boost lipid production. This work assessed the intrinsic ability of and to produce high amounts of microbial oils from these unusual carbon sources. Results demonstrated that minor differences in SCFA concentration (only 2 g/L) had a significant effect on yeast growth and lipid production. A C:N of 80 promoted yeast growth at all SCFA concentrations and favored SCFA consumption at 19 g/L SCFAs. The different SCFA uptake preferences in and highlighted the importance of considering the SCFA profile to select a suitable yeast strain for microbial oils production. At the most challenging SCFA concentration (19 g/L), 57.2% ±1.6% (w/w) and 78.4 ± 0.6% (w/w) lipid content were obtained in and , respectively. These values are among the highest reported for wild-type strains. To circumvent the challenges associated with media with high nitrogen content, this report also proved struvite precipitation as an effective method for increasing lipid production (from 17.9 ± 3.9% (w/w) to 41.9 ± 2.6% (w/w)) after nitrogen removal in food waste-derived media.
可以利用短链脂肪酸 (SCFA) 作为碳源积累微生物油脂。富含 SCFA 的培养基通常含有大量的氮，这会阻止提高产油所需的高碳氮比 (C:N)。本研究评估了 和 从这些不寻常的碳源生产大量微生物油脂的内在能力。结果表明，SCFA 浓度的微小差异（仅 2g/L）对酵母生长和油脂生产有显著影响。在所有 SCFA 浓度下，C:N 为 80 促进酵母生长，并有利于 19g/L SCFA 下 SCFA 的消耗。 和 对不同 SCFA 吸收偏好的强调，突出了在选择适合微生物油脂生产的酵母菌株时考虑 SCFA 谱的重要性。在最具挑战性的 SCFA 浓度（19g/L）下， 和 分别获得了 57.2%±1.6%（w/w）和 78.4%±0.6%（w/w）的油脂含量。这些值是野生型菌株中报道的最高值之一。为了避免高氮含量培养基带来的挑战，本报告还证明了鸟粪石沉淀是一种有效的方法，可以在去除氮源后从废食物来源的培养基中提高油脂产量（从 17.9%±3.9%（w/w）增加到 41.9%±2.6%（w/w））。
