微小RNA-204的上调通过下调SOX4抑制人肾癌细胞的增殖、迁移和侵袭。
Upregulation of microRNA-204 inhibits cell proliferation, migration and invasion in human renal cell carcinoma cells by downregulating SOX4.
作者信息
Wu Deyao, Pan Huixing, Zhou Yunfeng, Zhang Zichun, Qu Ping, Zhou Jian, Wang Wanxiang
机构信息
Department of Urology, The Fourth Affiliated Hospital of Nantong Medical College, Yancheng City No. 1 People's Hospital, Yancheng, Jiangsu 224001, P.R. China.
Inspection Branch, The Fourth Affiliated Hospital of Nantong Medical College, Yancheng City No. 1 People's Hospital, Yancheng, Jiangsu 224001, P.R. China.
出版信息
Mol Med Rep. 2015 Nov;12(5):7059-64. doi: 10.3892/mmr.2015.4259. Epub 2015 Aug 27.
MicroRNA-204 (miR-204) has been reported to be frequently downregulated in various types of cancer, including renal, brain, ovary, hematological and colon cancer. The present study, investigated the effects of miR‑204 on renal cell carcinoma. Following transfection of miR‑204, an MTT assay, cell migration assay, cell invasion assay, western blot analysis and luciferase assay were performed in renal cell carcinoma cell lines. It was demonstrated that miR‑204 inhibits cell proliferation, migration and invasion in 786‑O and A498 cells. To the best of our knowledge, this study is the first to demonstrate that miR‑204 directly targets SOX4 in renal cell carcinoma. These results suggested that miR-204 may have value as a marker for the early detection of tumor metastasis and a therapeutic target preventing the invasion of renal cell carcinoma.
据报道，微小RNA-204（miR-204）在包括肾癌、脑癌、卵巢癌、血液系统癌症和结肠癌在内的多种类型癌症中经常下调。本研究调查了miR-204对肾细胞癌的影响。在肾癌细胞系中进行miR-204转染后，进行了MTT试验、细胞迁移试验、细胞侵袭试验、蛋白质印迹分析和荧光素酶试验。结果表明，miR-204抑制786-O和A498细胞的增殖、迁移和侵袭。据我们所知，本研究首次证明miR-204在肾细胞癌中直接靶向SOX4。这些结果表明，miR-204可能具有作为肿瘤转移早期检测标志物和预防肾细胞癌侵袭的治疗靶点的价值。
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