通过免疫组织化学观察果蝇卵泡细胞中的细胞蛇表达
Visualizing Cytoophidia Expression in Drosophila Follicle Cells via Immunohistochemistry.
作者信息
Tastan Ömür Y, Liu Ji-Long
机构信息
MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, OX1 3PT, UK.
出版信息
Methods Mol Biol. 2015;1328:179-89. doi: 10.1007/978-1-4939-2851-4_13.
We describe a user-friendly immunohistochemical approach for the detection of protein localization in Drosophila ovaries, here focusing on CTP synthase. This approach mainly uses fluorescently labeled antibodies to detect single, double, or multiple antigens. We provide a step-by-step protocol with detailed notes and tips, a simplified method that can also be adapted to detect protein localization beyond Drosophila ovaries.
我们描述了一种用于检测果蝇卵巢中蛋白质定位的用户友好型免疫组织化学方法，这里重点介绍CTP合酶。该方法主要使用荧光标记抗体来检测单一、双重或多种抗原。我们提供了一份带有详细注释和提示的分步方案，这是一种简化方法，也可适用于检测果蝇卵巢以外的蛋白质定位。
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