新型膜装置及其在干血浆斑分析前血样采集中的潜在应用。
Novel membrane devices and their potential utility in blood sample collection prior to analysis of dried plasma spots.
作者信息
Sturm Robert, Henion Jack, Abbott Richard, Wang Phil
机构信息
Q2 Solutions, 19 Brown Road, Ithaca, NY 14850, USA.
Bayer Pharma AG, Bioanalytics & Non-Clinical Pharmacokinetics, 42096 Wuppertal, Germany.
出版信息
Bioanalysis. 2015;7(16):1987-2002. doi: 10.4155/bio.15.98.
BACKGROUND
Construction and application of a novel membrane substrate dried plasma spot (DPS) card is described. Results/methodology: The online automation compatible prototype DPS card employs a membrane filter to remove red blood cells from whole blood for the collection of plasma. The described autoDPS card provides acceptable quantitative precision and accuracy data from plasma filtered from 45% hematocrit whole blood. When significantly lower or higher hematocrit (30 and 60%) whole blood fortified with guanfacine is applied, the quantitative precision and accuracy data fall outside regulated 15/20 bioanalytical acceptance criteria.
CONCLUSION
The described prototype DPS card works well for normal hematocrit whole blood, but further development is needed for samples of much lower or higher hematocrit.
背景
描述了一种新型膜基质干血浆斑（DPS）卡的构建与应用。结果/方法：该在线自动化兼容的原型DPS卡采用膜滤器从全血中去除红细胞以收集血浆。所描述的自动DPS卡从血细胞比容为45%的全血中过滤得到的血浆提供了可接受的定量精密度和准确度数据。当应用血细胞比容显著更低或更高（30%和60%）且添加了胍法辛的全血时，定量精密度和准确度数据超出了规定的15/20生物分析可接受标准。
结论
所描述的原型DPS卡对于正常血细胞比容的全血效果良好，但对于血细胞比容低得多或高得多的样本还需要进一步改进。
Zotero 插件