斑点42 RNA：一种在中心代谢中发挥作用的调控性小RNA。
The Spot 42 RNA: A regulatory small RNA with roles in the central metabolism.
作者信息
Bækkedal Cecilie, Haugen Peik
机构信息
a Department of Chemistry ; The Norwegian Structural Biology Center (NorStruct) and Center for Bioinformatics (SfB); UiT - The Arctic University of Norway ; Tromsø , Norway.
出版信息
RNA Biol. 2015;12(10):1071-7. doi: 10.1080/15476286.2015.1086867. Epub 2015 Sep 1.
Abstract
The Spot 42 RNA is a 109 nucleotide long (in Escherichia coli) noncoding small regulatory RNA (sRNA) encoded by the spf (spot fourty-two) gene. spf is found in gamma-proteobacteria and the majority of experimental work on Spot 42 RNA has been performed using E. coli, and recently Aliivibrio salmonicida. In the cell Spot 42 RNA plays essential roles as a regulator in carbohydrate metabolism and uptake, and its expression is activated by glucose, and inhibited by the cAMP-CRP complex. Here we summarize the current knowledge on Spot 42, and present the natural distribution of spf, show family-specific secondary structural features of Spot 42, and link highly conserved structural regions to mRNA target binding.
摘要
斑点42 RNA是一种由spf（斑点42）基因编码的长度为109个核苷酸（在大肠杆菌中）的非编码小调控RNA（sRNA）。spf存在于γ-变形菌中，关于斑点42 RNA的大多数实验工作是使用大肠杆菌进行的，最近也使用了杀鲑气单胞菌。在细胞中，斑点42 RNA作为碳水化合物代谢和摄取的调节剂发挥着重要作用，其表达被葡萄糖激活，并被cAMP-CRP复合物抑制。在这里，我们总结了关于斑点42的当前知识，展示了spf的自然分布，呈现了斑点42的家族特异性二级结构特征，并将高度保守的结构区域与mRNA靶标结合联系起来。
相似文献
1
The Spot 42 RNA: A regulatory small RNA with roles in the central metabolism.斑点42 RNA：一种在中心代谢中发挥作用的调控性小RNA。
RNA Biol. 2015;12(10):1071-7. doi: 10.1080/15476286.2015.1086867. Epub 2015 Sep 1.
2
Gifsy-1 Prophage IsrK with Dual Function as Small and Messenger RNA Modulates Vital Bacterial Machineries.具有小RNA和信使RNA双重功能的Gifsy-1原噬菌体IsrK调节重要的细菌机制。
PLoS Genet. 2016 Apr 8;12(4):e1005975. doi: 10.1371/journal.pgen.1005975. eCollection 2016 Apr.
3
Expression profiling reveals Spot 42 small RNA as a key regulator in the central metabolism of Aliivibrio salmonicida.表达谱分析揭示 Spot 42 小 RNA 是鲑鱼气单胞菌中心代谢的关键调节因子。
BMC Genomics. 2012 Jan 24;13:37. doi: 10.1186/1471-2164-13-37.
4
The target spectrum of SdsR small RNA in Salmonella.沙门氏菌中SdsR小RNA的靶标谱。
Nucleic Acids Res. 2016 Dec 1;44(21):10406-10422. doi: 10.1093/nar/gkw632. Epub 2016 Jul 12.
5
Mechanistic study of base-pairing small regulatory RNAs in bacteria.细菌中碱基配对小调控RNA的机制研究
Methods. 2017 Mar 15;117:67-76. doi: 10.1016/j.ymeth.2016.09.012. Epub 2016 Sep 28.
6
Superfolder GFP reporters validate diverse new mRNA targets of the classic porin regulator, MicF RNA.超折叠 GFP 报告基因验证了经典孔蛋白调节因子 MicF RNA 的多种新 mRNA 靶标。
Mol Microbiol. 2012 May;84(3):428-45. doi: 10.1111/j.1365-2958.2012.08031.x. Epub 2012 Mar 28.
7
Absolute Regulatory Small Noncoding RNA Concentration and Decay Rates Measurements in Escherichia coli.大肠杆菌中绝对调控性小非编码RNA浓度及衰减率的测量
Methods Mol Biol. 2018;1737:231-248. doi: 10.1007/978-1-4939-7634-8_14.
8
Thermodynamic aspects of the self-assembly of DsrA, a small noncoding RNA from Escherichia coli.大肠杆菌小非编码RNA DsrA自组装的热力学方面
Acta Biochim Pol. 2014;61(1):179-84. Epub 2014 Jan 22.
9
DsrA regulatory RNA represses both hns and rbsD mRNAs through distinct mechanisms in Escherichia coli.DsrA调控RNA通过不同机制在大肠杆菌中抑制hns和rbsD mRNA。
Mol Microbiol. 2015 Oct;98(2):357-69. doi: 10.1111/mmi.13129. Epub 2015 Sep 4.
10
Systematic characterization of artificial small RNA-mediated inhibition of Escherichia coli growth.人工小RNA介导的大肠杆菌生长抑制的系统表征
RNA Biol. 2017 Feb;14(2):206-218. doi: 10.1080/15476286.2016.1270001. Epub 2016 Dec 16.
引用本文的文献
1
Overview of small proteins encoded by bacterial dual-function small RNAs.细菌双功能小RNA编码的小蛋白概述。
Noncoding RNA Res. 2025 Jul 6;15:44-50. doi: 10.1016/j.ncrna.2025.07.002. eCollection 2025 Dec.
2
Advances in sRNA-mediated regulation of infection in the host.宿主中sRNA介导的感染调控研究进展。
Front Cell Infect Microbiol. 2025 May 15;15:1503337. doi: 10.3389/fcimb.2025.1503337. eCollection 2025.
3
Marine bacteria spp. require UDP-glucose-4-epimerase for aggregation and production of sticky exopolymer.海洋细菌 spp. 需要 UDP-葡萄糖-4-差向异构酶来进行聚集和产生粘性胞外聚合物。
mBio. 2024 Aug 14;15(8):e0003824. doi: 10.1128/mbio.00038-24. Epub 2024 Jul 3.
4
Improved RNA stability estimation through Bayesian modeling reveals most transcripts have subminute half-lives.通过贝叶斯建模提高 RNA 稳定性估计，揭示大多数转录本的半衰期都不足一分钟。
Proc Natl Acad Sci U S A. 2024 Apr 2;121(14):e2308814121. doi: 10.1073/pnas.2308814121. Epub 2024 Mar 25.
5
Structural Basis of Dual Specificity of Sinorhizobium meliloti Clr, a cAMP and cGMP Receptor Protein.苜蓿中华根瘤菌 Clr 蛋白的双重特异性的结构基础，一种 cAMP 和 cGMP 受体蛋白。
mBio. 2023 Apr 25;14(2):e0302822. doi: 10.1128/mbio.03028-22. Epub 2023 Apr 5.
6
Central Role of Sibling Small RNAs NgncR_162 and NgncR_163 in Main Metabolic Pathways of Neisseria gonorrhoeae.Sibling Small RNAs NgncR_162 和 NgncR_163 在淋病奈瑟菌主要代谢途径中的核心作用。
mBio. 2023 Feb 28;14(1):e0309322. doi: 10.1128/mbio.03093-22. Epub 2023 Jan 4.
7
Synthetic Genetic Interactions Reveal a Dense and Cryptic Regulatory Network of Small Noncoding RNAs in Escherichia coli.合成遗传相互作用揭示了大肠杆菌中小非编码 RNA 的密集且隐匿的调控网络。
mBio. 2022 Aug 30;13(4):e0122522. doi: 10.1128/mbio.01225-22. Epub 2022 Aug 3.
8
Dual-function Spot 42 RNA encodes a 15-amino acid protein that regulates the CRP transcription factor.双功能 Spot 42 RNA 编码一个 15 个氨基酸的蛋白质，该蛋白调节 CRP 转录因子。
Proc Natl Acad Sci U S A. 2022 Mar 8;119(10):e2119866119. doi: 10.1073/pnas.2119866119. Epub 2022 Mar 3.
9
CsrA regulation via binding to the base-pairing small RNA Spot 42.通过与碱基配对的小 RNA Spot 42 结合对 CsrA 进行调节。
Mol Microbiol. 2022 Jan;117(1):32-53. doi: 10.1111/mmi.14769. Epub 2021 Jul 7.
10
Spot 42 RNA regulates putrescine catabolism in Escherichia coli by controlling the expression of puuE at the post-transcription level.Spot 42 RNA 通过在后转录水平控制 puuE 的表达来调节大肠杆菌中的腐胺分解代谢。
J Microbiol. 2021 Feb;59(2):175-185. doi: 10.1007/s12275-021-0421-4. Epub 2021 Feb 1.
本文引用的文献
1
Two-level inhibition of galK expression by Spot 42: Degradation of mRNA mK2 and enhanced transcription termination before the galK gene.Spot 42对galK表达的两级抑制：mRNA mK2的降解以及galK基因之前转录终止的增强。
Proc Natl Acad Sci U S A. 2015 Jun 16;112(24):7581-6. doi: 10.1073/pnas.1424683112. Epub 2015 Jun 4.
2
Post-transcriptional regulation of the virulence-associated enzyme AlgC by the σ(22) -dependent small RNA ErsA of Pseudomonas aeruginosa.铜绿假单胞菌 σ(22) 依赖型小 RNA ErsA 对毒力相关酶 AlgC 的转录后调控。
Environ Microbiol. 2015 Jan;17(1):199-214. doi: 10.1111/1462-2920.12590. Epub 2014 Sep 3.
3
Evolutionary patterns of Escherichia coli small RNAs and their regulatory interactions.大肠杆菌小 RNA 的进化模式及其调控相互作用。
RNA. 2014 Jul;20(7):994-1003. doi: 10.1261/rna.043133.113. Epub 2014 May 27.
4
Small RNA sX13: a multifaceted regulator of virulence in the plant pathogen Xanthomonas.小分子 RNA sX13：植物病原菌黄单胞菌毒力的多功能调控因子。
PLoS Pathog. 2013 Sep;9(9):e1003626. doi: 10.1371/journal.ppat.1003626. Epub 2013 Sep 12.
5
Rfam 11.0: 10 years of RNA families.RFAM 11.0：10 年的 RNA 家族。
Nucleic Acids Res. 2013 Jan;41(Database issue):D226-32. doi: 10.1093/nar/gks1005. Epub 2012 Nov 3.
6
Multiple factors dictate target selection by Hfq-binding small RNAs.多种因素决定了 Hfq 结合小 RNA 的靶标选择。
EMBO J. 2012 Apr 18;31(8):1961-74. doi: 10.1038/emboj.2012.52. Epub 2012 Mar 2.
7
Expression profiling reveals Spot 42 small RNA as a key regulator in the central metabolism of Aliivibrio salmonicida.表达谱分析揭示 Spot 42 小 RNA 是鲑鱼气单胞菌中心代谢的关键调节因子。
BMC Genomics. 2012 Jan 24;13:37. doi: 10.1186/1471-2164-13-37.
8
Interactive Tree Of Life v2: online annotation and display of phylogenetic trees made easy.交互式生命树 v2：轻松在线注释和显示系统发育树
Nucleic Acids Res. 2011 Jul;39(Web Server issue):W475-8. doi: 10.1093/nar/gkr201. Epub 2011 Apr 5.
9
The base-pairing RNA spot 42 participates in a multioutput feedforward loop to help enact catabolite repression in Escherichia coli.碱基配对 RNA 斑点 42 参与多输出前馈回路，有助于在大肠杆菌中实施分解代谢物阻遏。
Mol Cell. 2011 Feb 4;41(3):286-97. doi: 10.1016/j.molcel.2010.12.027.
10
The Sinorhizobium meliloti RNA chaperone Hfq influences central carbon metabolism and the symbiotic interaction with alfalfa.苜蓿中华根瘤菌 RNA 伴侣蛋白 Hfq 影响中心碳代谢和与紫花苜蓿的共生相互作用。
BMC Microbiol. 2010 Mar 6;10:71. doi: 10.1186/1471-2180-10-71.
Zotero 插件