He Jinxi, Fang Yu, Chen Xiaoxin
Department of Thoracic Surgery, Ningxia Medical University General Hospital; Cancer Research Program, North Carolina Central University.
Cancer Research Program, North Carolina Central University; Department of Thoracic & Cardiovascular Surgery, The First Affiliated Hospital of Chongqing Medical University;
J Vis Exp. 2015 Aug 25(102):e53012. doi: 10.3791/53012.
Multiple surgical procedures have been reported to induce gastroesophageal reflux in animals. Herein, we report three surgical models with mice aiming to induce reflux of gastric contents, duodenal contents or mixed contents. Surgical procedures and general principles have been described in detail. A researcher with surgical experience should be able to grasp the technique after a short period of practice. After surgery, most mice can survive and develop reflux esophagitis similar to that in humans. However, it should be noted that histological differences between mouse and human esophagus are the inherent limitations of these surgical models. If used for research on Barrett's esophagus and adenocarcinoma, these procedures may need to be combined with genetic modifications.
据报道，多种外科手术可在动物中诱发胃食管反流。在此，我们报告三种针对小鼠的手术模型，旨在诱发胃内容物、十二指肠内容物或混合内容物的反流。手术步骤和一般原则已详细描述。有手术经验的研究人员经过短时间练习应能掌握该技术。手术后，大多数小鼠能够存活并发展出与人类相似的反流性食管炎。然而，应注意小鼠和人类食管之间的组织学差异是这些手术模型的固有局限性。如果用于巴雷特食管和腺癌的研究，这些手术可能需要与基因改造相结合。
