Zhang Zhenzhen, Atwell Lauren L, Farris Paige E, Ho Emily, Shannon Jackilen
1School of Public Health,Oregon Health & Science University,3181 SW Sam Jackson Park Road,MC: GH153,Portland,OR 97239,USA.
2School of Biological and Population Health Sciences,Oregon State University,Corvallis,OR,USA.
Public Health Nutr. 2016 May;19(7):1288-95. doi: 10.1017/S136898001500244X. Epub 2015 Sep 2.
To examine the relationship between dietary cruciferous vegetable intake and selected tumour biomarkers for histone acetylation (H3K9ac, H3K18ac, HDAC3 and HDAC6), proliferation (Ki-67) and cell-cycle regulation (p21) from breast tissue.
The study used baseline data of women recruited to participate in a clinical trial of sulforaphane supplement. Dietary cruciferous vegetable intake was collected through a validated Arizona Cruciferous Vegetable Intake Questionnaire. Breast tissue was obtained from biopsy samples. Spearman correlations were calculated between intake of specific cruciferous vegetables and biomarkers. Tissue biomarkers were log2-transformed to obtain approximate normality. Linear regression analyses were conducted to examine associations between cruciferous vegetable intake and biomarkers adjusting for age and use of non-steroidal anti-inflammatory drugs. False discovery rate (FDR) was used to account for multiple comparisons.
Clinical trial baseline.
Fifty-four women who had abnormal mammogram findings and were scheduled for breast biopsy.
Mean intake of total cruciferous vegetables from all food sources was 81·7 (sd 57·3) g/d. Mean urinary total sulforaphane metabolites was 0·08 (sd 0·07) µm/mm creatinine. Total cruciferous vegetable intake was inversely associated with Ki-67 protein expression in breast ductal carcinoma in situ (DCIS) tissue (β=-0·004; se=0·001; FDR q value=0·03), but not in benign or invasive ductal carcinoma (IDC) tissue. No association was found for other biomarkers measured (HDAC3, HDAC6, H3K9, H3K18 and p21) in all tissues examined (benign, DCIS and IDC).
The present study sought to provide additional evidence for the potential role of sulforaphane in histone acetylation and cell proliferation. Here, we report that total cruciferous vegetable intake is associated with decreased cell proliferation in breast DCIS tissue.
研究膳食十字花科蔬菜摄入量与乳腺组织中组蛋白乙酰化（H3K9ac、H3K18ac、HDAC3和HDAC6）、增殖（Ki-67）及细胞周期调控（p21）相关肿瘤生物标志物之间的关系。
本研究使用了参与萝卜硫素补充剂临床试验的女性的基线数据。通过经过验证的亚利桑那十字花科蔬菜摄入量问卷收集膳食十字花科蔬菜摄入量。乳腺组织取自活检样本。计算特定十字花科蔬菜摄入量与生物标志物之间的Spearman相关性。对组织生物标志物进行log2转换以获得近似正态分布。进行线性回归分析，以检验十字花科蔬菜摄入量与生物标志物之间的关联，并对年龄和非甾体抗炎药的使用情况进行校正。采用错误发现率（FDR）来处理多重比较。
临床试验基线。
54名乳房X光检查结果异常且计划进行乳腺活检的女性。
所有食物来源的十字花科蔬菜总平均摄入量为81.7（标准差57.3）克/天。尿中萝卜硫素代谢物总平均水平为0.08（标准差0.07）微摩尔/毫摩尔肌酐。十字花科蔬菜总摄入量与乳腺导管原位癌（DCIS）组织中的Ki-67蛋白表达呈负相关（β=-0.004；标准误=0.001；FDR q值=0.03），但在良性或浸润性导管癌（IDC）组织中无此相关性。在所检查的所有组织（良性、DCIS和IDC）中，未发现与其他测量的生物标志物（HDAC3、HDAC6、H3K9、H3K18和p21）存在关联。
本研究旨在为萝卜硫素在组蛋白乙酰化和细胞增殖中的潜在作用提供更多证据。在此，我们报告十字花科蔬菜总摄入量与乳腺DCIS组织中细胞增殖减少有关。
