白刺叶提取物抑制黑色素瘤细胞生长并增强B16F10黑色素瘤细胞黑色素生成的植物化学能力
Phytochemical capacity of Nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma.
作者信息
Boubaker Jihed, Mokdad Bzeouich Imen, Nasr Nouha, Ben Ghozlen Hajer, Mustapha Nadia, Ghedira Kamel, Chekir-Ghedira Leila
机构信息
Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Rue Avicenne, Monastir, 5000, Tunisia.
Unity of bioactive natural substances and biotechnology, Faculty of Pharmacy, University of Monastir, Rue Avicenne, Monastir, 5000, Tunisia.
出版信息
BMC Complement Altern Med. 2015 Sep 2;15:300. doi: 10.1186/s12906-015-0743-z.
BACKGROUND
Here, phytochemical profile of Nitraria retusa (N. Retusa) leaf extracts was identified and their ability to induce apoptosis and inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma was evaluated.
METHODS
The Apoptosis was evidenced by investigating DNA fragmentation, and Acridine orange/ethidium bromide staining. Amounts of melanin and tyrosinase were measured spectrophotometrically at 475 nm.
RESULTS
Extracts from Nitraria retusa exhibited significant anti-proliferative activity after 48 h of incubation. Our result was confirmed by ladder DNA fragmentation profile. All extracts showed also the ability to enhance melanogenesis and tyrosinase activity of B16F10 melanoma cells.
CONCLUSION
The tested extracts have a significant biological effect which may be due to their bioactive compounds.
背景
在此，对白刺叶提取物的植物化学成分进行了鉴定，并评估了它们诱导黑色素瘤细胞凋亡、抑制其生长以及增强B16F10黑色素瘤细胞黑色素生成的能力。
方法
通过研究DNA片段化以及吖啶橙/溴化乙锭染色来证明细胞凋亡。在475nm处用分光光度法测量黑色素和酪氨酸酶的含量。
结果
白刺提取物在孵育48小时后表现出显著的抗增殖活性。梯形DNA片段化图谱证实了我们的结果。所有提取物还显示出增强B16F10黑色素瘤细胞黑色素生成和酪氨酸酶活性的能力。
结论
所测试的提取物具有显著的生物学效应，这可能归因于其生物活性化合物。
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