Huang He, Wang Yan, An Yang, Tian Yaguang, Li Shu, Teng Xiaohua
College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Environ Sci Pollut Res Int. 2017 Jun;24(16):14312-14321. doi: 10.1007/s11356-017-9019-0. Epub 2017 Apr 20.
Lead (Pb) is a toxic element and environmental pollutant. Pb toxicity and antagonistic effect of selenium (Se) on Pb have been deeply studied in mammals. The testis is one of the target organs of Pb in birds. The aim of this study was to investigate the mitigating effect of Se on Pb toxicity in chicken testes by determining messenger RNA (mRNA) expressions of 5 heat shock proteins (HSPs) and 25 selenoproteins. Sixty male chickens (7-day-old) were randomly divided into the control group, the Se group, the Pb group, and the Pb + Se group, and were fed for 90 days. The feeding methods of chickens were as follows: The control group was fed drinking water and commercial diet (0.49 mg/kg Se). Lead acetate was added into the drinking water (350 mg/L Pb). Sodium selenite was added into the commercial diet (1 mg/kg Se). Multivariate correlation analysis and principal component analysis (PCA) were used to define the relationships among all the measured factors and the most important parameters that could be used as key factors, respectively. The results indicated that Se decreased the increase of mRNA expressions of all the HSPs and increased the decrease of mRNA expressions of all the selenoproteins induced by Pb in the chicken testes. HSP70 may be a biomarker of Pb poisoning in the chicken testes. Se alleviated Pb-induced toxicity in the chicken testes through regulating mRNA expressions of HSPs and selenoproteins.
铅（Pb）是一种有毒元素和环境污染物。在哺乳动物中，铅的毒性以及硒（Se）对铅的拮抗作用已得到深入研究。睾丸是鸟类体内铅的靶器官之一。本研究的目的是通过测定5种热休克蛋白（HSPs）和25种硒蛋白的信使核糖核酸（mRNA）表达，来研究硒对鸡睾丸中铅毒性的缓解作用。将60只7日龄雄性鸡随机分为对照组、硒组、铅组和铅+硒组，并饲养90天。鸡的饲养方法如下：对照组给予饮用水和商业饲料（硒含量为0.49 mg/kg）。在饮用水中添加醋酸铅（铅含量为350 mg/L）。在商业饲料中添加亚硒酸钠（硒含量为1 mg/kg）。分别采用多变量相关分析和主成分分析（PCA）来确定所有测量因素之间的关系以及可作为关键因素的最重要参数。结果表明，硒降低了鸡睾丸中所有热休克蛋白mRNA表达的增加，并增加了铅诱导的所有硒蛋白mRNA表达的降低。HSP70可能是鸡睾丸中铅中毒的生物标志物。硒通过调节热休克蛋白和硒蛋白的mRNA表达来减轻铅对鸡睾丸的毒性。
