Lin Yun-Peng, Jiang Rong-Cai, Zhang Jian-Ning
Department of Neurosurgery, Tianjin Medical University General Hospital; Tianjin Neurological Institute; Key Laboratory of Post-trauma Neurorepair and Regeneration in Central Nervous System, Ministry of Education; Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, Tianjin, China ; Tianjin Medical University General Hospital, Tianjin, China.
Department of Neurosurgery, Tianjin Medical University General Hospital; Tianjin Neurological Institute; Key Laboratory of Post-trauma Neurorepair and Regeneration in Central Nervous System, Ministry of Education; Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, Tianjin, China.
Neural Regen Res. 2015 Jul;10(7):1088-94. doi: 10.4103/1673-5374.160100.
Fluid percussion-induced traumatic brain injury models have been widely used in experimental research for years. In an experiment, the stability of impaction is inevitably affected by factors such as the appearance of liquid spikes. Management of impact pressure is a crucial factor that determines the stability of these models, and direction of impact control is another basic element. To improve experimental stability, we calculated a pressure curve by generating repeated impacts using a fluid percussion device at different pendulum angles. A stereotactic frame was used to control the direction of impact. We produced stable and reproducible models, including mild, moderate, and severe traumatic brain injury, using the MODEL01-B device at pendulum angles of 6°, 11° and 13°, with corresponding impact force values of 1.0 ± 0.11 atm (101.32 ± 11.16 kPa), 2.6 ± 0.16 atm (263.44 ± 16.21 kPa), and 3.6 ± 0.16 atm (364.77 ± 16.21 kPa), respectively. Behavioral tests, hematoxylin-eosin staining, and magnetic resonance imaging revealed that models for different degrees of injury were consistent with the clinical properties of mild, moderate, and severe craniocerebral injuries. Using this method, we established fluid percussion models for different degrees of injury and stabilized pathological features based on precise power and direction control.
多年来，流体冲击诱导的创伤性脑损伤模型已在实验研究中广泛应用。在一项实验中，冲击的稳定性不可避免地受到诸如液体尖峰出现等因素的影响。冲击压力的控制是决定这些模型稳定性的关键因素，而冲击控制方向是另一个基本要素。为了提高实验稳定性，我们通过使用流体冲击装置在不同摆角下产生重复冲击来计算压力曲线。使用立体定向框架来控制冲击方向。我们使用MODEL01 - B装置在摆角为6°、11°和13°时分别产生了稳定且可重复的模型，包括轻度、中度和重度创伤性脑损伤，相应的冲击力值分别为1.0±0.11 atm（101.32±11.16 kPa）、2.6±0.16 atm（263.44±16.21 kPa）和3.6±0.16 atm（364.77±16.21 kPa）。行为测试、苏木精 - 伊红染色和磁共振成像显示，不同程度损伤的模型与轻度、中度和重度颅脑损伤的临床特征相符。使用这种方法，我们基于精确的功率和方向控制建立了不同程度损伤的流体冲击模型，并稳定了病理特征。
