Afzalzadeh Mohammad Reza, Ahangarpour Akram, Amirzargar Ashraf, Varnamkhasti Mohammad Kazemi, Ganjalidarani Hadi
Department of Veterinary, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Health Research Institute, Diabetes Research Center, Department of Physiology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
World J Mens Health. 2015 Aug;33(2):109-16. doi: 10.5534/wjmh.2015.33.2.109. Epub 2015 Aug 19.
Vitis vinifera is a species of Vitis that is native to the Mediterranean region, central Europe, and southwestern Asia, and has been used as a drug in traditional medicine. Traditional medicinal plants have been used for medical purposes with increasing effectiveness. It is important to identify drugs that inhibit spermatogenesis. Therefore, the present study aimed to investigate the effect of grape juice (GJ) on serum levels of inhibin B and sperm count in normal male rats.
Thirty-five adult male rats were randomly divided into five groups, each containing seven rats. Rats in the control group received 1 mL of normal saline over the course of the study. The experimental groups received GJ (100, 200, 400, and 1,600 mg/kg, orally, for 35 days consecutively). At the end of the treatment period, fertility indices were measured, including body weight difference, sex organ weight, sperm motility and count, epididymal sperm reserve, daily sperm production (DSP), and serum inhibin B levels.
We found that GJ reduces body weight difference, was associated with decreased sperm motility and count in all treatment groups (p≤0.05 and p≤0.001, respectively). Moreover, DSP was significantly decreased in all treatment groups compared to the control group (p≤0.05), except in the group receiving 100 mg/kg of GJ. Inhibin B levels were significantly decreased in all treatment groups (p≤0.05).
The results of our study suggest that GJ in all doses, but especially in higher doses, may decrease fertility in male rats.
葡萄（Vitis vinifera）是葡萄属的一个物种，原产于地中海地区、中欧和亚洲西南部，在传统医学中被用作药物。传统药用植物在医学用途上的有效性日益提高。识别抑制精子发生的药物很重要。因此，本研究旨在探讨葡萄汁（GJ）对正常雄性大鼠血清抑制素B水平和精子数量的影响。
35只成年雄性大鼠随机分为五组，每组7只。对照组大鼠在研究过程中接受1 mL生理盐水。实验组大鼠连续35天口服GJ（100、200、400和1600 mg/kg）。在治疗期结束时，测量生育指标，包括体重差异、性器官重量、精子活力和数量、附睾精子储备、每日精子生成量（DSP）以及血清抑制素B水平。
我们发现GJ可降低体重差异，且在所有治疗组中均与精子活力和数量下降有关（分别为p≤0.05和p≤0.001）。此外，与对照组相比，除接受100 mg/kg GJ的组外，所有治疗组的DSP均显著降低（p≤0.05）。所有治疗组的抑制素B水平均显著降低（p≤0.05）。
我们的研究结果表明，所有剂量的GJ，尤其是高剂量的GJ，可能会降低雄性大鼠的生育能力。
