Zhao Dong-Jie, Chen Yang, Wang Zi-Yang, Xue Lin, Mao Tong-Lin, Liu Yi-Min, Wang Zhong-Yi, Huang Lan
College of Information and Electrical Engineering, China Agricultural University, Beijing 100083, China.
State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Sci Rep. 2015 Sep 3;5:13425. doi: 10.1038/srep13425.
The limitations of conventional extracellular recording and intracellular recording make high-resolution multisite recording of plant bioelectrical activity in situ challenging. By combining a cooled charge-coupled device camera with a voltage-sensitive dye, we recorded the action potentials in the stem of Helianthus annuus and variation potentials at multiple sites simultaneously with high spatial resolution. The method of signal processing using coherence analysis was used to determine the synchronization of the selected signals. Our results provide direct visualization of the phloem, which is the distribution region of the electrical activities in the stem and leaf of H. annuus, and verify that the phloem is the main action potential transmission route in the stems of higher plants. Finally, the method of optical recording offers a unique opportunity to map the dynamic bioelectrical activity and provides an insight into the mechanisms of long-distance electrical signal transmission in higher plants.
传统的细胞外记录和细胞内记录的局限性使得对植物生物电活动进行高分辨率的原位多部位记录具有挑战性。通过将冷却电荷耦合器件相机与电压敏感染料相结合，我们以高空间分辨率同时记录了向日葵茎中的动作电位和多个部位的变异电位。使用相干分析的信号处理方法来确定所选信号的同步性。我们的结果直接可视化了韧皮部，韧皮部是向日葵茎和叶中电活动的分布区域，并验证了韧皮部是高等植物茎中主要的动作电位传播途径。最后，光学记录方法为绘制动态生物电活动提供了独特的机会，并深入了解了高等植物中长距离电信号传输的机制。
